Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation.
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SUBMITTER: Cooper CJ
PROVIDER: S-EPMC7305189 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
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Structure determination of the HgcAB complex using metagenome sequence data: insights into microbial mercury methylation.
Cooper Connor J CJ Zheng Kaiyuan K Rush Katherine W KW Johs Alexander A Sanders Brian C BC Pavlopoulos Georgios A GA Kyrpides Nikos C NC Podar Mircea M Ovchinnikov Sergey S Ragsdale Stephen W SW Parks Jerry M JM
Communications biology 20200619 1
Bacteria and archaea possessing the hgcAB gene pair methylate inorganic mercury (Hg) to form highly toxic methylmercury. HgcA consists of a corrinoid binding domain and a transmembrane domain, and HgcB is a dicluster ferredoxin. However, their detailed structure and function have not been thoroughly characterized. We modeled the HgcAB complex by combining metagenome sequence data mining, coevolution analysis, and Rosetta structure calculations. In addition, we overexpressed HgcA and HgcB in Esch ...[more]
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