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IFN? Production Profile in Turkeys of Different Immunological Status after TRT Vaccination.


ABSTRACT: Introduction:Despite vaccination against avian metapneumoviruses (aMPV), cases of turkey rhinotracheitis (TRT) caused by aMPV field strains are frequently reported. Differences have been shown in the level of immune system stimulation after aMPV vaccination between turkeys that do and do not possess specific anti-aMPV maternally derived antibodies (MDA). The article describes the influence of MDA on the production of IFN? in the spleen of aMPV-vaccinated turkeys. Material and Methods:MDA+ or MDA- turkeys were vaccinated against TRT after hatching or on the 14th day of life. Spleen samples were collected 3, 7, and 14 days post vaccination for mononuclear cell isolation. Real-time PCR, flow cytometry, and the enzyme-linked immunospot assay were used to evaluate the levels of IFN? gene expression, production, and secretion by cells within the spleen samples. Results:Increased IFN? gene expression was noticed after vaccination only in birds that did not possess MDA or possessed MDA at relatively low level (MDA+ birds vaccinated at 14 DOL). In all birds, an increased percentage of T lymphocytes producing IFN? was recorded. The proportion of anti-aMPV IFN?-secreting cells was increased only in MDA- birds. Conclusion:Besides having a protective role, MDA are known to interfere with vaccination efficacy. The analysis of our results confirms that MDA can decrease the level of immune system stimulation after aMPV vaccination of turkeys.

SUBMITTER: Smialek M 

PROVIDER: S-EPMC7305653 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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IFNγ Production Profile in Turkeys of Different Immunological Status after TRT Vaccination.

Śmiałek Marcin M   Tykałowski Bartłomiej B   Dziewulska Daria D   Kowalczyk Joanna J   Koncicki Andrzej A  

Journal of veterinary research 20200616 2


<h4>Introduction</h4>Despite vaccination against avian metapneumoviruses (aMPV), cases of turkey rhinotracheitis (TRT) caused by aMPV field strains are frequently reported. Differences have been shown in the level of immune system stimulation after aMPV vaccination between turkeys that do and do not possess specific anti-aMPV maternally derived antibodies (MDA). The article describes the influence of MDA on the production of IFNγ in the spleen of aMPV-vaccinated turkeys.<h4>Material and methods<  ...[more]

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