Ontology highlight
ABSTRACT:
SUBMITTER: Marek M
PROVIDER: S-EPMC7306515 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Marek Martin M Chaloupkova Radka R Prudnikova Tatyana T Sato Yukari Y Rezacova Pavlina P Nagata Yuji Y Kuta Smatanova Ivana I Damborsky Jiri J
Computational and structural biotechnology journal 20200603
Engineering enzyme catalytic properties is important for basic research as well as for biotechnological applications. We have previously shown that the reshaping of enzyme access tunnels via the deletion of a short surface loop element may yield a haloalkane dehalogenase variant with markedly modified substrate specificity and enantioselectivity. Here, we conversely probed the effects of surface loop-helix transplantation from one enzyme to another within the enzyme family of haloalkane dehaloge ...[more]