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Identification of Adenosine-to-Inosine RNA Editing with Acrylonitrile Reagents.

ABSTRACT: New chemical probes have been designed to facilitate the identification of adenosine-to-inosine (A-to-I) edited RNAs. These reagents combine a conjugate acceptor for selective inosine covalent modification with functional groups for bioorthogonal biotinylation. The resulting biotinylated RNA was enriched and verified with RT-qPCR. This powerful chemical approach provides new opportunities to identify and quantify A-to-I editing sites.

SUBMITTER: Li Y

PROVIDER: S-EPMC7308179 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Identification of Adenosine-to-Inosine RNA Editing with Acrylonitrile Reagents.

Li Ying Y Göhl Matthias M Ke Ke K Vanderwal Christopher D CD Spitale Robert C RC

Organic letters 20190913 19

New chemical probes have been designed to facilitate the identification of adenosine-to-inosine (A-to-I) edited RNAs. These reagents combine a conjugate acceptor for selective inosine covalent modification with functional groups for bioorthogonal biotinylation. The resulting biotinylated RNA was enriched and verified with RT-qPCR. This powerful chemical approach provides new opportunities to identify and quantify A-to-I editing sites. ...[more]

PMID: 31516001

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Identification of Diverse Adenosine-to-Inosine RNA Editing Subtypes in Colorectal Cancer.

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Increased adenosine-to-inosine RNA editing in rheumatoid arthritis.

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Adenosine-to-inosine RNA editing shapes transcriptome diversity in primates.

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Proteogenomics of adenosine-to-inosine RNA editing in fruit fly

Project description:This dataset is a part of a bigger work on RNA editing in D. melanogaster brain. We present Orbitrap spectra searched against a customized database of Drosophila proteome with introduction of variants provided by RNA editing. Tree files reflect 3 technical replicates.

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Proteogenomics of adenosine-to-inosine RNA editing in fruit fly

2018-10-25 | PXD009590 | Pride

Testicular adenosine to inosine RNA editing in the mouse is mediated by ADARB1.

Project description:Adenosine to inosine (A-to-I) RNA editing occurs in a wide range of tissues and cell types and can be catalyzed by one of the two adenosine deaminase acting on double-stranded RNA enzymes, ADAR and ADARB1. Editing can impact both coding and noncoding regions of RNA, and in higher organisms has been proposed to function in adaptive evolution. Neither the prevalence of A-to-I editing nor the role of either ADAR or ADARB1 has been examined in the context of germ cell development in mammals. Computational analysis of whole testis and cell-type specific RNA-sequencing data followed by molecular confirmation demonstrated that A-to-I RNA editing occurs in both the germ line and in somatic Sertoli cells in two targets, Cog3 and Rpa1. Expression analysis demonstrated both Adar and Adarb1 were expressed in both Sertoli cells and in a cell-type dependent manner during germ cell development. Conditional ablation of Adar did not impact testicular RNA editing in either germ cells or Sertoli cells. Additionally, Adar ablation in either cell type did not have gross impacts on germ cell development or male fertility. In contrast, global Adarb1 knockout animals demonstrated a complete loss of A-to-I RNA editing in spite of normal germ cell development. Taken together, these observations demonstrate ADARB1 mediates A-to-I RNA editing in the testis and these editing events are dispensable for male fertility in an inbred mouse strain in the lab.

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