Ontology highlight
ABSTRACT:
SUBMITTER: Kwon T
PROVIDER: S-EPMC7309224 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Kwon Taehong T Ko Sung Hee SH Hamel Jean-François P JP Han Jongyoon J
Analytical chemistry 20200324 7
We demonstrate a new micro/nanofluidic system for continuous and automatic monitoring of protein product size and quantity directly from the culture supernatant during a high-cell-concentration CHO cell perfusion culture. A microfluidic device enables clog-free cell retention for a bench-scale (350 mL) perfusion bioreactor that continuously produces the culture supernatant containing monoclonal antibodies (IgG<sub>1</sub>). A nanofluidic device directly monitors the protein size and quantity in ...[more]