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Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein.

ABSTRACT: In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins and the anionic surfaces, with concomitant counterion release. Hence, we anticipate that this novel "green" approach of forming cushioned bilayers can be an important tool to mimic the cell membrane without the disturbance of the solid substrate, thereby achieving a further understanding of protein-cell interactions.

SUBMITTER: Gerelli Y

PROVIDER: S-EPMC7311080 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein.

Gerelli Yuri Y Eriksson Skog Amanda A Jephthah Stephanie S Welbourn Rebecca J L RJL Klechikov Alexey A Skepö Marie M

Langmuir : the ACS journal of surfaces and colloids 20200407 15

In this article, it is shown that by exposing commonly used lipids for biomembrane mimicking studies, to a solution containing the histidine-rich intrinsically disordered protein histatin 5, a protein cushion spontaneously forms underneath the bilayer. The underlying mechanism is attributed to have an electrostatic origin, and it is hypothesized that the observed behavior is due to proton charge fluctuations promoting attractive electrostatic interactions between the positively charged proteins ...[more]

PMID: 32212610

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