Ontology highlight
ABSTRACT:
SUBMITTER: Ghergurovich JM
PROVIDER: S-EPMC7311271 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Ghergurovich Jonathan M JM García-Cañaveras Juan C JC Wang Joshua J Schmidt Emily E Zhang Zhaoyue Z TeSlaa Tara T Patel Harshel H Chen Li L Britt Emily C EC Piqueras-Nebot Marta M Gomez-Cabrera Mari Carmen MC Lahoz Agustín A Fan Jing J Beier Ulf H UH Kim Hahn H Rabinowitz Joshua D JD
Nature chemical biology 20200511 7
Glucose is catabolized by two fundamental pathways, glycolysis to make ATP and the oxidative pentose phosphate pathway to make reduced nicotinamide adenine dinucleotide phosphate (NADPH). The first step of the oxidative pentose phosphate pathway is catalyzed by the enzyme glucose-6-phosphate dehydrogenase (G6PD). Here we develop metabolite reporter and deuterium tracer assays to monitor cellular G6PD activity. Using these, we show that the most widely cited G6PD antagonist, dehydroepiandosterone ...[more]