Browse
Submit Data
Databases
API
Help

Dataset Information

28 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Facile Histamine Detection by Surface-Enhanced Raman Scattering using SiO₂@Au@Ag Alloy Nanoparticles.

ABSTRACT: Histamine intoxication associated with seafood consumption represents a global health problem. The consumption of high concentrations of histamine can cause illnesses ranging from light symptoms, such as a prickling sensation, to death. In this study, gold-silver alloy-embedded silica (SiO₂@Au@Ag) nanoparticles were created to detect histamine using surface-enhanced Raman scattering (SERS). The optimal histamine SERS signal was measured following incubation with 125 μg/mL of SiO₂@Au@Ag for 2 h, with a material-to-histamine solution volume ratio of 1:5 and a phosphate-buffered saline-Tween 20 (PBS-T) solvent at pH 7. The SERS intensity of the histamine increased proportionally with the increase in histamine concentration in the range 0.1-0.8 mM, with a limit of detection of 3.698 ppm. Our findings demonstrate the applicability of SERS using nanomaterials for histamine detection. In addition, this study demonstrates that nanoalloys could have a broad application in the future.

SUBMITTER: Huynh KH

PROVIDER: S-EPMC7311956 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Plasmonic Au@Ag@mSiO2 Nanorattles for In Situ Imaging of Bacterial Metabolism by Surface-Enhanced Raman Scattering Spectroscopy.

Project description:It is well known that microbial populations and their interactions are largely influenced by their secreted metabolites. Noninvasive and spatiotemporal monitoring and imaging of such extracellular metabolic byproducts can be correlated with biological phenotypes of interest and provide new insights into the structure and development of microbial communities. Herein, we report a surface-enhanced Raman scattering (SERS) hybrid substrate consisting of plasmonic Au@Ag@mSiO2 nanorattles for optophysiological monitoring of extracellular metabolism in microbial populations. A key element of the SERS substrate is the mesoporous silica shell encapsulating single plasmonic nanoparticles, which furnishes colloidal stability and molecular sieving capabilities to the engineered nanostructures, thereby realizing robust, sensitive, and reliable measurements. The reported SERS-based approach may be used as a powerful tool for deciphering the role of extracellular metabolites and physicochemical factors in microbial community dynamics and interactions.

| S-EPMC8719315 | biostudies-literature

Large Scale Solid-state Synthesis of Catalytically Active Fe3O4@M (M = Au, Ag and Au-Ag alloy) Core-shell Nanostructures.

Project description:Solvent-less synthesis of nanostructures is highly significant due to its economical, eco-friendly and industrially viable nature. Here we report a solid state synthetic approach for the fabrication of Fe3O4@M (where M?=?Au, Ag and Au-Ag alloy) core-shell nanostructures in nearly quantitative yields that involves a simple physical grinding of a metal precursor over Fe3O4 core, followed by calcination. The process involves smooth coating of low melting hybrid organic-inorganic precursor over the Fe3O4 core, which in turn facilitates a continuous shell layer post thermolysis. The obtained core-shell nanostructures are characterized using, XRD, XPS, ED-XRF, FE-SEM and HR-TEM for their phase, chemical state, elemental composition, surface morphology, and shell thickness, respectively. Homogeneous and continuous coating of the metal shell layer over a large area of the sample is ascertained by SAXS and STEM analyses. The synthesized catalysts have been studied for their applicability towards a model catalytic hydrogen generation from NH3BH3 and NaBH4 as hydrogen sources. The catalytic efficacy of the Fe3O4@Ag and Ag rich alloy shell materials are found to be superior to the corresponding Au counterparts. The saturation magnetization studies reveal the potential of the core-shell nanostructured catalysts to be magnetically recoverable and recyclable.

| S-EPMC6488626 | biostudies-literature

Tuning interior nanogaps of double-shelled Au/Ag nanoboxes for surface-enhanced Raman scattering.

Project description:Double-shelled Au/Ag hollow nanoboxes with precisely controlled interior nanogaps (1 to 16?nm) were synthesized for gap-tunable surface-enhanced Raman scattering (SERS). The double-shelled nanoboxes were prepared via a two-step galvanic replacement reaction approach using Ag nanocubes as the templates, while 4-aminothiolphenol (4-ATP) as SERS probe molecules were loaded between the two shells. More than 10-fold enhancement of SERS is observed from the double-shelled nanoboxes than Ag nanocubes. In addition, the SERS of the double-shelled nanoboxes increase significantly with the decrease of gap size, consistent with the theoretical prediction that smaller gap size induces larger localized electromagnetic enhancement.

| S-EPMC4323660 | biostudies-literature

Thermally Stable TiO2 - and SiO2 -Shell-Isolated Au Nanoparticles for In Situ Plasmon-Enhanced Raman Spectroscopy of Hydrogenation Catalysts.

Project description:Raman spectroscopy is known as a powerful technique for solid catalyst characterization as it provides vibrational fingerprints of (metal) oxides, reactants, and products. It can even become a strong surface-sensitive technique by implementing shell-isolated surface-enhanced Raman spectroscopy (SHINERS). Au@TiO2 and Au@SiO2 shell-isolated nanoparticles (SHINs) of various sizes were therefore prepared for the purpose of studying heterogeneous catalysis and the effect of metal oxide coating. Both SiO2 - and TiO2 -SHINs are effective SHINERS substrates and thermally stable up to 400?°C. Nano-sized Ru and Rh hydrogenation catalysts were assembled over the SHINs by wet impregnation of aqueous RuCl3 and RhCl3 . The substrates were implemented to study CO adsorption and hydrogenation under in situ conditions at various temperatures to illustrate the differences between catalysts and shell materials with SHINERS. This work demonstrates the potential of SHINS for in situ characterization studies in a wide range of catalytic reactions.

| S-EPMC5873377 | biostudies-literature

Gd2O3-doped silica @ Au nanoparticles for in vitro imaging cancer biomarkers using surface-enhanced Raman scattering.

Project description:There has been an interest in developing multimodal approaches to combine the advantages of individual imaging modalities, as well as to compensate for respective weaknesses. We previously reported a composite nano-system composed of gadolinium-doped mesoporous silica nanoparticle and gold nanoparticle (Gd-Au NPs) as an efficient MRI contrast agent for in vivo cancer imaging. However, MRI lacks sensitivity and is unsuitable for in vitro cancer detection. Thus, here we performed a study to use the Gd-Au NPs for detection and imaging of a widely recognized human cancer biomarker, epidermal growth factor receptor (EGFR), in individual human cancer cells with surface-enhanced Raman scattering (SERS). The Gd-Au NPs were sequentially conjugated with a monoclonal antibody recognizing EGFR and a Raman reporter molecule, 4-meraptobenzoic acid (MBA), to generate a characteristic SERS signal at 1075cm-1. By spatially mapping the SERS intensity at 1075cm-1, cellular distribution of EGFR and its relocalization on the plasma membrane were measured in situ. In addition, the EGFR expression levels in three human cancer cell lines (S18, A431 and A549) were measured using this SERS probe, which were consistent with the comparable measurements using immunoblotting and immunofluorescence. Our SERS results show that functionalized Gd-Au NPs successfully targeted EGFR molecules in three human cancer cell lines and monitored changes in single cell EGFR distribution in situ, demonstrating its potential to study cell activity under physiological conditions. This SERS study, combined with our previous MRI study, suggests the Gd-Au nanocomposite is a promising candidate contrast agent for multimodal cancer imaging.

| S-EPMC5427483 | biostudies-literature

Raman amplification in the ultra-small limit of Ag nanoparticles on SiO2 and graphene: Size and inter-particle distance effects.

Project description:Size, shape and hot spots are crucial to optimize Raman amplification from metallic nanoparticle (NPs). The amplification from radius = 1.8 ± 0.4 nm ultra-small silver NPs was explored. Increasing NP density redshifts and widens their plasmon that, according to simulations for NPs arrays, is originated by the reduction of the interparticle distance, d, becoming remarkable for d ≤ R. Inter-particle interaction red-shifts (N130 nm) and widens (N90 nm) the standard plasmon of non-interacting spherical particles. Graphene partly delocalizes the carriers enhancing the NIR spectral weight. Raman amplification of graphene phonons is moderate and depends smoothly on d while that of Rhodamine 6G (R6G) varies almost exponentially due to their location at hotspots that depend strongly on d. The experimental correlation between amplification and plasmon position is well reproduced by simulations. The amplification originated by the ultra-small NPs is compared to that of larger particles, granular silver films with 7 < R < 15 nm grains, with similar extinction values. The amplification is found to be larger for the 1.8nm NPs due to the higher surface/volume ration that allows higher density of hot spots. It is demonstrated that Raman amplification can be efficiently increased by depositing low density layers of ultra-small NPs on top of granular films.

| S-EPMC7116317 | biostudies-literature

Raman scattering of linear chains of strongly coupled Ag nanoparticles on SWCNTs.

Project description:We compare the Raman scattering properties of hybrid nanostructures consisting of Ag nanoparticles (NPs) in disordered and aligned arrangements on single-walled carbon nanotubes (SWCNTs) as a result of chemical and photoreduction methods. In the latter case, the unique structure of the very small Ag NP (from 4 to 7 nm) chains generated an extremely large mode at 969 cm(-1) that was assigned to the sulphate-silver interaction at the NP surface. Another strong mode was present at 1201 cm(-1) and was assigned to an IR-active mode of sodium dodecyl sulphate (SDS); this mode was observed because the symmetry changes altered the selection rules. We demonstrate that both the UV photoreduction of silver and the presence of SWCNTs are necessary to produce this very strong Raman scattering. The Raman modes of the SWCNTs are also significantly modified by the presence of Ag NP chains along the nanotubes.

| S-EPMC4050379 | biostudies-literature

Toxicity of TiO2, SiO2, ZnO, CuO, Au and Ag engineered nanoparticles on hatching and early nauplii of Artemia sp.

Project description:The potential of environmental release enhances with increased commercial applications of the nanomaterials. In this work, a simple and efficient test to estimate the acute toxicity of nanoparticles is carried out on Artemia species and their hatching rates. We have tested six different engineered nanoparticles (silver, gold, copper oxide, zinc oxide, TiO2 and SiO2 nanoparticles) and three soluble salts (CuSO4, ZnSO4 and AgNO3) on Artemia sp. The physicochemical properties of the nanoparticles involved in this study were analyzed in normal water and marine water. Hydrated and bleached Artemia cysts were allowed to hatch in continuously aerated, filtered sterile salt water containing nanoparticles; hatching of viable nauplii and total hatchlings have been recorded. In parallel, standard Artemia toxicity test was conducted on the nauplii monitoring the viability. In hatching experiments, a reduction in hatching rate was observed along with mortality of newly hatched nauplii. The results of the hatching experiment and of the standard Artemia test showed a good correlation. The toxicity of the nanoparticles was compared and the order of toxicity was estimated as Ag>CuO>ZnO>Au>TiO2>SiO2. The study thus suggests that the hatching test itself is a reliable assay for determining the toxicity of nanomaterials.

| S-EPMC6321756 | biostudies-literature

Ag@Au core-shell dendrites: a stable, reusable and sensitive surface enhanced Raman scattering substrate.

Project description:Surface enhanced Raman scattering (SERS) substrate based on fabricated Ag@Au core-shell dendrite was achieved. Ag dendrites were grown on Si wafer by the hydrothermal corrosion method and Au nanofilm on the surface of Ag dendritic nanostructure was then fabricated by chemical reduction. With the help of sodium borohydride in water, Au surface absorbates such as thiophene, adenine, rhodamine, small anions (Br(-) and I(-)), and a polymer (PVP, poly(N-vinylpyrrolidone)) can be completely and rapidly removed. After four repeatable experiments, the substrate SERS function did not decrease at all, indicating that the Ag@Au dendrite should be of great significance to SERS application because it can save much resource. Six-month-duration stability tests showed that the Ag@Au core-shell dendrite substrate is much more stable than the Ag dendrite substrates. We have also experimented on fast detection of Cd(2+) at 10(-8) M concentration by decorating single-stranded DNA containing adenine and guanine bases on the surface of this Ag@Au dendrite. Finite-difference time-domain simulations were carried out to investigate the influence of Au nanolayer on Ag dendrites, which showed that the local electric fields and enhancement factor are hardly affected when a 4 nm Au nanolayer is coated on Ag dendrite surface.

| S-EPMC4585979 | biostudies-literature

Seed-Mediated Growth of Ag@Au Nanodisks with Improved Chemical Stability and Surface-Enhanced Raman Scattering.

Project description:Bimetallic Ag@Au nanoparticles (NPs) have received significant research interest because of their unique optical properties and molecular sensing ability through surface-enhanced Raman scattering (SERS). However, the synthesis of Ag@Au core-shell plasmonic nanostructures with precisely controlled size and shape remained a great challenge. Here, we report a simple approach for the synthesis of bimetallic Ag@Au nanodisks of about 13.5 nm thickness and different diameters through a seed-mediated growth process. The synthesis involves the conformal deposition of Au atoms at the corner sites of Ag nanoplate (AgNPL) seeds coupled with site-selective oxidative etching of AgNPL edges to generate Ag@Au nanodisks. The resultant Ag@Au nanodisks manifest significantly improved chemical stability and tunable localized surface plasmon resonance from the visible to the near-infrared spectral range. Moreover, in comparison to AgNPLs, the Ag@Au nanodisks showed greatly enhanced SERS performance with an enhancement factor up to 0.47 × 105, which is nearly 3-fold higher than that of the original AgNPLs (0.18 × 105). Furthermore, the Ag@Au nanodisks show a high sensitivity for detecting probe molecules such as crystal violet of concentration as low as 10-9 M and excellent reproducibility, with the SERS intensity fluctuation less than 12.5%. The synthesis route adapted for the controlled fabrication of Ag@Au nanodisks can be a potential platform for maneuvering other bimetallic plasmonic nanostructures useful for plasmonics and sensing applications.

| S-EPMC6644819 | biostudies-literature