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The Desaturase Gene Nlug-desatA2 Regulates the Performance of the Brown Planthopper Nilaparvata lugens and Its Relationship with Rice.

ABSTRACT: Insect desaturases are known to play an important role in chemical communication between individuals. However, their roles in insect growth, development and fecundity, and in regulating interactions of insects with plants, remain largely unknown. In this study, we explored the functions of Nlug-desatA2, a desaturase gene of the brown planthopper (BPH), Nilaparvata lugens (Stål). The RNA interference-based knockdown of Nlug-desatA2 decreased the ratio of monounsaturated fatty acids to saturated fatty acids, and the level of fatty acids and triglycerides in BPH. Nlug-desatA2-knockdown also reduced the food intake, body mass and fecundity of female BPH adults, and led to abdomen atrophy and ovarian agenesis. Nlug-desatA2-knockdown suppressed the transcription of TOR (target of rapamycin), Lpp (Lipophorin) and AKHR (adipokinetic hormone receptor) in female adults. Moreover, the corrected survival rate of BPH with Nlug-desatA2-knockdown fed an artificial diet was higher than the survival rate of those fed on rice plants. Higher levels of salicylic acid in rice infested by Nlug-desatA2-knockdown female BPH adults than in rice infested by control BPH may be the reason. These findings demonstrate that Nlug-desatA2 has an essential role in lipid metabolism and is involved in the food intake, survival, development and fecundity of BPH. In addition, this gene is likely involved in regulating the responses of rice to BPH infestation.

SUBMITTER: Ye W

PROVIDER: S-EPMC7312190 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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The Desaturase Gene Nlug-desatA2 Regulates the Performance of the Brown Planthopper Nilaparvata lugens and Its Relationship with Rice.

Ye Wenfeng W Zeng Jiamei J Hu Wenhui W Bustos-Segura Carlos C Noman Ali A Lou Yonggen Y

International journal of molecular sciences 20200610 11

Insect desaturases are known to play an important role in chemical communication between individuals. However, their roles in insect growth, development and fecundity, and in regulating interactions of insects with plants, remain largely unknown. In this study, we explored the functions of Nlug-desatA2, a desaturase gene of the brown planthopper (BPH), Nilaparvata lugens (Stål). The RNA interference-based knockdown of Nlug-desatA2 decreased the ratio of monounsaturated fatty ...[more]

PMID: 32532001

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Project description:BACKGROUND: The brown planthopper (BPH) Nilaparvata lugens (Stål) is one of the most serious insect pests of rice in Asia. However, little is known about the mechanisms responsible for the development, wing dimorphism and sex difference in this species. Genomic information for BPH is currently unavailable, and, therefore, transcriptome and expression profiling data for this species are needed as an important resource to better understand the biological mechanisms of BPH. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we performed de novo transcriptome assembly and gene expression analysis using short-read sequencing technology (Illumina) combined with a tag-based digital gene expression (DGE) system. The transcriptome analysis assembles the gene information for different developmental stages, sexes and wing forms of BPH. In addition, we constructed six DGE libraries: eggs, second instar nymphs, fifth instar nymphs, brachypterous female adults, macropterous female adults and macropterous male adults. Illumina sequencing revealed 85,526 unigenes, including 13,102 clusters and 72,424 singletons. Transcriptome sequences larger than 350 bp were subjected to Gene Orthology (GO) and KEGG Orthology (KO) annotations. To analyze the DGE profiling, we mainly compared the gene expression variations between eggs and second instar nymphs; second and fifth instar nymphs; fifth instar nymphs and three types of adults; brachypterous and macropterous female adults as well as macropterous female and male adults. Thousands of genes showed significantly different expression levels based on the various comparisons. And we randomly selected some genes to confirm their altered expression levels by quantitative real-time PCR (qRT-PCR). CONCLUSIONS/SIGNIFICANCE: The obtained BPH transcriptome and DGE profiling data provide comprehensive gene expression information at the transcriptional level that could facilitate our understanding of the molecular mechanisms from various physiological aspects including development, wing dimorphism and sex difference in BPH.

Dataset Information

The Desaturase Gene Nlug-desatA2 Regulates the Performance of the Brown Planthopper Nilaparvata lugens and Its Relationship with Rice.

Publications

The Desaturase Gene <i>Nlug-desatA2</i> Regulates the Performance of the Brown Planthopper <i>Nilaparvata lugens</i> and Its Relationship with Rice.

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