Unknown

Dataset Information

0

Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study.


ABSTRACT: One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgrade the difficulty of biological mixing following shotgun mass spectrometry. To benchmark the proposed pipeline, we examined heterogeneity of the HepG2 hepatoblastoma cell line proteome. Data are available via ProteomeXchange with identifier PXD018450.

SUBMITTER: Kiseleva O 

PROVIDER: S-EPMC7312985 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Empowering Shotgun Mass Spectrometry with 2DE: A HepG2 Study.

Kiseleva Olga O   Ponomarenko Elena E   Poverennaya Ekaterina E  

International journal of molecular sciences 20200527 11


One of the major goals of the Chromosome-Centric Human Proteome Project (C-HPP) is to catalog and annotate a myriad of heterogeneous proteoforms, produced by ca. 20 thousand genes. To achieve a detailed and personalized understanding into proteomes, we suggest using a customized RNA-seq library of potential proteoforms, which includes aberrant variants specific to certain biological samples. Two-dimensional electrophoresis coupled with high-performance liquid chromatography allowed us to downgra  ...[more]

Similar Datasets

| S-EPMC2897201 | biostudies-literature
| S-EPMC6800818 | biostudies-literature
| S-EPMC2798817 | biostudies-literature
| S-EPMC2597439 | biostudies-literature
| S-EPMC4716738 | biostudies-other
| S-EPMC7083916 | biostudies-literature
| S-EPMC4474766 | biostudies-literature
| S-EPMC3134881 | biostudies-literature
| S-EPMC2650121 | biostudies-literature
| S-EPMC4970217 | biostudies-literature