Ontology highlight
ABSTRACT:
SUBMITTER: Yang X
PROVIDER: S-EPMC7313891 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
DNA repair 20200622
MacroD1 is an enzyme that hydrolyzes protein mono-ADP-ribosylation. However, the key catalytic residues of MacroD1 in these biochemical reactions remain elusive. Here, we present the crystal structure of MacroD1 in a complex with ADP-ribose (ADPR). The β5-α10-loop functions as a switch loop to mediate substrate recognition and right orientation. The conserved Phe<sup>272</sup> in the β5-α10-loop plays a crucial role in the orientation of ADPR distal ribose, and a conserved hydrogen-bond network ...[more]