Dataset Information

MicroRNA-296-5p is differentially expressed in individuals with and without HIV-1 infection.

ABSTRACT: MicroRNAs are considered as potential biomarkers, agents, or therapeutic targets; few studies have addressed the expression of miRNAs in treatment-naïve patients infected with HIV-1. The aim of this study was to assess plasma relative circulating miRNA expression profiles in treatment-naïve Mexican patients with HIV/AIDS and healthy individuals using a commercial array. A low CD4+ T cell count and high viral load were found in all patients. Decreased relative miRNA-296-5p expression was observed in patients; moreover, this was the only miRNA that showed differences between the two groups. Thus, we measured the absolute expression of miR-296-5p by qPCR, confirming the result with statistically significant differences (P < 0.05). There is evidence that miR-296-5p regulates the expression of the PIN1 gene, which encodes the peptidylprolyl Cis/Trans isomerase NIMA-Interacting-1, that is involved in different stages of the biological cycle of HIV-1, this relationship is corroborated by bioinformatics analysis and ELISA assay was used to measure plasma levels of PIN1. The decreased expression of miR-296-5p found in naïve patients with HIV infection suggests a regulatory activity of this miRNA on virus replication, making it a potential therapeutic agent against HIV. Finally, miR-296-5p could be inhibiting the virus transcription by regulating genes different than PIN1.

SUBMITTER: Cardenas-Bedoya J

PROVIDER: S-EPMC7315763 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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MicroRNA-296-5p is differentially expressed in individuals with and without HIV-1 infection.

Cárdenas-Bedoya Jhonathan J Marquez-Pedroza Jazmin J Morán-Moguel María Cristina MC Escoto-Delgadillo Martha M Vázquez-Valls Eduardo E González-Enríquez Gracia Viviana GV Pérez-Ríos Alma Minerva AM Torres-Mendoza Blanca Miriam BM

Genetics and molecular biology 20200622 3

MicroRNAs are considered as potential biomarkers, agents, or therapeutic targets; few studies have addressed the expression of miRNAs in treatment-naïve patients infected with HIV-1. The aim of this study was to assess plasma relative circulating miRNA expression profiles in treatment-naïve Mexican patients with HIV/AIDS and healthy individuals using a commercial array. A low CD4+ T cell count and high viral load were found in all patients. Decreased relative miRNA-296-5p expression was observed ...[more]

PMID: 32584920

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Dataset Information

MicroRNA-296-5p is differentially expressed in individuals with and without HIV-1 infection.

Publications

MicroRNA-296-5p is differentially expressed in individuals with and without HIV-1 infection.

Similar Datasets

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