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DES2 is a fatty acid ?11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis.

ABSTRACT: Arbuscular mycorrhizal (AM) fungi are oleaginous organisms, and the most abundant fatty acyl moiety usually found in their lipids is palmitvaccenic acid (16:1?11cis ). However, it is not known how this uncommon fatty acid species is made. Here, we have cloned two homologues of lepidopteran fatty acyl-coenzyme A ?11 desaturases from the AM fungus Rhizophagus irregularis. Both enzymes, DES1 and DES2, are expressed in intraradical mycelium and can complement the unsaturated fatty acid-requiring auxotrophic growth phenotype of the Saccharomyces cerevisiae ole1? mutant. DES1 expression leads almost exclusively to oleic acid (18:1?9cis ) production, whereas DES2 expression results in the production of 16:1?11cis and vaccenic acid (18:1?11cis ). DES2 therefore encodes a ?11 desaturase that is likely to be responsible for the synthesis of 16:1?11cis in R. irregularis.

SUBMITTER: Cheeld H

PROVIDER: S-EPMC7317563 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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DES2 is a fatty acid Δ11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis.

Cheeld Henry H Bhutada Govindprasad G Beaudoin Frederic F Eastmond Peter J PJ

FEBS letters 20200303 11

Arbuscular mycorrhizal (AM) fungi are oleaginous organisms, and the most abundant fatty acyl moiety usually found in their lipids is palmitvaccenic acid (16:1Δ11cis ). However, it is not known how this uncommon fatty acid species is made. Here, we have cloned two homologues of lepidopteran fatty acyl-coenzyme A Δ11 desaturases from the AM fungus Rhizophagus irregularis. Both enzymes, DES1 and DES2, are expressed in intraradical mycelium and can complement the unsaturated fatty acid-re ...[more]

PMID: 32060917

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