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Leishmanicidal Activity of an In Silico-Screened Novel Inhibitor against Ascorbate Peroxidase of Leishmania donovani.


ABSTRACT: Peroxidases are a heterogeneous family of enzymes that have diverse biological functions. Ascorbate peroxidase is a redox enzyme that is reduced by trypanothione, which plays a central role in the redox defense system of Leishmania In view of developing new and novel therapeutics, we performed in silico studies in order to search for a ligand library and identify new drug candidates and their physiological roles against promastigotes and intracellular amastigotes of Leishmania donovani Our results demonstrated that the selected inhibitor ZINC96021026 has significant antileishmanial effect and effectively killed both free and intracellular forms of the parasite. ZINC96021026 was found to be identical to ML-240, a selective inhibitor of valosin-containing protein (VCP), or p97, a member of the AAA-ATPase protein family which was derived from the scaffold of N 2,N 4-dibenzylquinazoline-2,4-diamine (DBeQ), targeting the D2-ATPase domain of the enzyme. ZINC96021026 (ML-240) thus has a broad range of cellular functions, thought to be derived from its ability to unfold proteins or disassemble protein complexes, besides inhibiting the ascorbate peroxidase activity. ML-240 may inhibit the parasite's ascorbate peroxidase, leading to extensive apoptosis and inducing generation of reactive oxygen species. Taken together, our results demonstrated that ML-240 could be an attractive therapeutic option for treatment against leishmaniasis.

SUBMITTER: Kashif M 

PROVIDER: S-EPMC7318017 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Leishmanicidal Activity of an <i>In Silico</i>-Screened Novel Inhibitor against Ascorbate Peroxidase of Leishmania donovani.

Kashif Mohammad M   Paladhi Ankush A   Singh Ranjeet R   Bhattacharyya Sankar S   Hira Sumit Kumar SK   Manna Partha Pratim PP  

Antimicrobial agents and chemotherapy 20200623 7


Peroxidases are a heterogeneous family of enzymes that have diverse biological functions. Ascorbate peroxidase is a redox enzyme that is reduced by trypanothione, which plays a central role in the redox defense system of <i>Leishmania</i> In view of developing new and novel therapeutics, we performed <i>in silico</i> studies in order to search for a ligand library and identify new drug candidates and their physiological roles against promastigotes and intracellular amastigotes of <i>Leishmania d  ...[more]

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