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Association of CRISPR/Cas System with the Drug Resistance in Klebsiella pneumoniae.


ABSTRACT: Background:Klebsiella pneumoniae is a common opportunistic pathogen and its production of extended-spectrum ?-lactamases (ESBL) and carbapenemases leads to drug resistance. Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated genes (Cas) are widespread in the genome of many bacteria and are a defense mechanism against foreign invaders such as plasmids and viruses. Purpose:To investigate the prevalence of the CRISPR/Cas system in wild type strains of K. pneumoniae in the hospital and its association with drug resistance. Materials and Methods:A total of 136 strains were collected and characterized their susceptibility to antimicrobial agents. The prevalence of CRISPR/Cas system was detected by PCR and DNA sequencing was analyzed by CRISPRFinder. The statistical analysis of the results was performed by SPSS. Results:We found that 50/136 (37%) isolates produced ESBL and 30/136 (22%) isolates were resistant to carbapenems. These isolates were liable to be multidrug resistant against ?-lactams, quinolones, and aminoglycosides. Among the carbapenem-resistant isolates, blaKPC was the main drug resistance-associated gene and different types of ESBL and AmpC genes were present. Resistance to ?-lactams, quinolones, aminoglycosides, tetracyclines, and ?-lactams/enzyme inhibitor were higher in absence of the CRISPR/Cas system. Eighteen spacers within the CRISPR arrays matched with the genomes of plasmids or phages, some of which carried drug resistance genes. Conclusion:ESBL-producing and carbapenem-resistant K. pneumoniae are more likely to develop multidrug resistance and show an inverse correlation between drug resistance and CRISPR/Cas system. Absence of CRISPR/Cas modules allow for the acquisition of external drug resistance genes.

SUBMITTER: Wang G 

PROVIDER: S-EPMC7320894 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Association of CRISPR/Cas System with the Drug Resistance in <i>Klebsiella pneumoniae</i>.

Wang Gang G   Song Guobin G   Xu Yuanhong Y  

Infection and drug resistance 20200623


<h4>Background</h4><i>Klebsiella pneumoniae</i> is a common opportunistic pathogen and its production of extended-spectrum β-lactamases (ESBL) and carbapenemases leads to drug resistance. Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated genes (Cas) are widespread in the genome of many bacteria and are a defense mechanism against foreign invaders such as plasmids and viruses.<h4>Purpose</h4>To investigate the prevalence of the CRISPR/Cas system in wild type  ...[more]

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