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In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations.


ABSTRACT: The air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 µg/mL, whereas the DPPH assay showed IC50 = 0.89 mg/mL, and the ABTS assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC-FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.

SUBMITTER: Okur ME 

PROVIDER: S-EPMC7321211 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of <i>Phlomis russeliana</i> Extract Gel Formulations.

Okur Mehmet Evren ME   Karadağ Ayşe Esra AE   Üstündağ Okur Neslihan N   Özhan Yağmur Y   Sipahi Hande H   Ayla Şule Ş   Daylan Benay B   Demirci Betül B   Demirci Fatih F  

Molecules (Basel, Switzerland) 20200610 11


The air-dried aerial parts of <i>Phlomis russeliana</i> (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by <i>n</i>-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of <i>P. russeliana</i> extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the <i>P. russeliana</i> methanol extract showed LOX inhibitory activity at IC<sub>50</sub> = 23.2 µg/mL, whereas the DPPH<sup>•</sup> assay showed IC<sub>50</  ...[more]

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