Dataset Information

[MiR-204 inhibits invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1].

ABSTRACT:

Objective

To investigate the effect of miR-204 on the invasion and metastasis of breast cancer by targeted regulation of HNRNPA2B1.

Methods

The bioinformatics database was used to obtain data of the expressions of miR-204 in breast cancer patients and the survival rate of the patients. RT-qPCR was used to detect the expression of miR-204 in breast cancer cell lines. The expression vector GV369-miR-204 was used to overexpress miR-204 in MDA-MB-231 cells. Transwell assay was performed to detect the effect of miR-204 on the migration and invasion ability of the breast cancer cells. The key genes (hub genes) of miR-204 were determined by bioinformatics method. A dual luciferase assay was used to analyze the targeting relationship between miR-204 and HNRNPA2B1. The expression of HNRNPA2B1 in MDA-MB-231 cells after miR-204 overexpression was detected by Western blotting, and Transwell assay was used to examine the changes in the cell invasion ability.

Results

The expression of miR-204 was decreased in both breast cancer tissues, and was significantly lower in breast cancer MDA-MB-231 cells than in MCF-10A cells (P < 0.05). The decreased expression of miR-204 was associated with poorer prognosis of breast cancer patients (P < 0.05). Upregulation of miR-204 in MDA-MB-231 cells significantly inhibited the invasion and migration of the cells (P < 0.05). Analysis of the data from the Starbase revealed that the expression of miR-204-5p was negatively correlated with the expression of HNRNPA2B1, and the expression of HNRNPA2B1 was increased in breast cancer patients (P < 0.05) in association with a poorer prognosis of the patients (P < 0.05). Dual luciferase assay demonstrated that miR-204 could bind to HNRNPA2B1 in a target-specific manner. Western blotting and Transwell assay showed that miR-204 significant inhibited the migration and invasion ability of breast cancer cells by targeting HNRNPA2B1 (P < 0.05).

Conclusions

miR-204 expression is decreased in breast cancer tissues and cells, and its overexpression can inhibit the invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1.

SUBMITTER: Zhang L

PROVIDER: S-EPMC7321266 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

[MiR-204 inhibits invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1].

Zhang Liping L Bai Jun J Hu Yaqiong Y Zhou Dandan D Zheng Quan Q Yin Chonggao C Mu Qingjie Q Li Hongli H

Nan fang yi ke da xue xue bao = Journal of Southern Medical University 20200601 6

<h4>Objective</h4>To investigate the effect of miR-204 on the invasion and metastasis of breast cancer by targeted regulation of HNRNPA2B1.<h4>Methods</h4>The bioinformatics database was used to obtain data of the expressions of miR-204 in breast cancer patients and the survival rate of the patients. RT-qPCR was used to detect the expression of miR-204 in breast cancer cell lines. The expression vector GV369-miR-204 was used to overexpress miR-204 in MDA-MB-231 cells. Transwell assay was perform ...[more]

PMID: 32895200

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Dataset Information

[MiR-204 inhibits invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1].

Objective

Methods

Results

Conclusions

Publications

[MiR-204 inhibits invasion and metastasis of breast cancer cells by targeted regulation of HNRNPA2B1].

Similar Datasets

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