Employing NaChBac for cryo-EM analysis of toxin action on voltage-gated Na⁺ channels in nanodisc.

ABSTRACT: NaChBac, the first bacterial voltage-gated Na⁺ (Na_v) channel to be characterized, has been the prokaryotic prototype for studying the structure-function relationship of Na_v channels. Discovered nearly two decades ago, the structure of NaChBac has not been determined. Here we present the single particle electron cryomicroscopy (cryo-EM) analysis of NaChBac in both detergent micelles and nanodiscs. Under both conditions, the conformation of NaChBac is nearly identical to that of the potentially inactivated Na_vAb. Determining the structure of NaChBac in nanodiscs enabled us to examine gating modifier toxins (GMTs) of Na_v channels in lipid bilayers. To study GMTs in mammalian Na_v channels, we generated a chimera in which the extracellular fragment of the S3 and S4 segments in the second voltage-sensing domain from Na_v1.7 replaced the corresponding sequence in NaChBac. Cryo-EM structures of the nanodisc-embedded chimera alone and in complex with HuwenToxin IV (HWTX-IV) were determined to 3.5 and 3.2 Å resolutions, respectively. Compared to the structure of HWTX-IV-bound human Na_v1.7, which was obtained at an overall resolution of 3.2 Å, the local resolution of the toxin has been improved from ?6 to ?4 Å. This resolution enabled visualization of toxin docking. NaChBac can thus serve as a convenient surrogate for structural studies of the interactions between GMTs and Na_v channels in a membrane environment.

SUBMITTER: Gao S 

PROVIDER: S-EPMC7322032 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature