MicroRNA-30a-5p^me: a novel diagnostic and prognostic biomarker for clear cell renal cell carcinoma in tissue and urine samples.

ABSTRACT:

Background

The rising incidence of renal cell carcinomas (RCC) constitutes a significant challenge owing to risk of overtreatment. Because aberrant microRNA (miR) promoter methylation contributes to cancer development, we investigated whether altered miR-30a-5p expression associates with DNA promoter methylation and evaluated the usefulness as clear cell RCC (ccRCC) diagnostic and prognostic markers.

Methods

Genome-wide methylome and RNA sequencing data from a set of ccRCC and normal tissue samples from The Cancer Genome Atlas (TCGA) database were integrated to identify candidate CpG loci involved in cancer onset. MiR-30a-5p expression and promoter methylation were quantitatively assessed by PCR in a tissue set (Cohort #1) and urine sets (Cohorts #2 and 3) from IPOPorto and

SUBMITTER: Outeiro-Pinho G 

PROVIDER: S-EPMC7323611 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature