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A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers.

ABSTRACT: Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA ligands was demonstrated using BePI and doxorubicin. Identification of triplex-interacting ligands may allow the regulation of genetic instability in human genomes.

SUBMITTER: Del Mundo IMA 

PROVIDER: S-EPMC7323859 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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A 'light-up' intercalator displacement assay for detection of triplex DNA stabilizers.

Del Mundo Imee M A IMA   Cho Eun Jeong EJ   Dalby Kevin N KN   Vasquez Karen M KM  

Chemical communications (Cambridge, England) 20200201 13

Here, we developed a coralyne-based, 'light-up' intercalator displacement assay to identify molecular stabilizers of triplex DNA using a sequence from a chromosomal breakpoint hotspot in the human c-MYC oncogene. Its potential to identify triplex DNA ligands was demonstrated using BePI and doxorubicin. Identification of triplex-interacting ligands may allow the regulation of genetic instability in human genomes. ...[more]

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