Ontology highlight
ABSTRACT:
SUBMITTER: Ramzy A
PROVIDER: S-EPMC7324556 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Ramzy Adam A Tudurí Eva E Glavas Maria M MM Baker Robert K RK Mojibian Majid M Fox Jessica K JK O'Dwyer Shannon M SM Dai Derek D Hu Xiaoke X Denroche Heather C HC Edeer Nazde N Gray Sarah L SL Verchere Cameron B CB Johnson James D JD Kieffer Timothy J TJ
Scientific reports 20200629 1
In vivo genetic manipulation is used to study the impact of gene deletion or re-expression on β-cell function and organism physiology. Cre-LoxP is a system wherein LoxP sites flanking a gene are recognized by Cre recombinase. Cre transgenic mice are the most prevalent technology used to deliver Cre but many models have caveats of off-target recombination, impaired β-cell function, and high cost of animal production. Inducible estrogen receptor conjugated Cre models face leaky recombination and c ...[more]