A novel bacterial β-N-acetyl glucosaminidase from Chitinolyticbacter meiyuanensis possessing transglycosylation and reverse hydrolysis activities.

ABSTRACT:

Background

N-Acetyl glucosamine (GlcNAc) and N-Acetyl chitooligosaccharides (N-Acetyl COSs) exhibit many biological activities, and have been widely used in the pharmaceutical, agriculture, food, and chemical industries. Particularly, higher N-Acetyl COSs with degree of polymerization from 4 to 7 ((GlcNAc)₄-(GlcNAc)₇) show good antitumor and antimicrobial activity, as well as possessing strong stimulating activity toward natural killer cells. Thus, it is of great significance to discover a β-N-acetyl glucosaminidase (NAGase) that can not only produce GlcNAc, but also synthesize N-Acetyl COSs.

Results

The gene encoding the novel β-N-acetyl glucosaminidase, designated CmNAGase, was cloned from Chi

SUBMITTER: Zhang A 

PROVIDER: S-EPMC7324980 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature