Dataset Information

Long-term Evolution and Remodeling of Soft Drusen in Rhesus Macaques.

ABSTRACT: Purpose:To characterize the evolution and structure of soft drusen in aged rhesus macaques using in vivo multimodal retinal imaging and ex vivo histologic and ultrastructural analyses as a nonhuman primate model of early age-related macular degeneration (AMD). Methods:Multimodal imaging including fundus photography, spectral domain optical coherence tomography (SD-OCT), and fundus autofluorescence (FAF) were used to characterize and track individual drusen lesions in 20 aged rhesus macaques (mean age 23.3 ± 2.7 years) with drusenoid lesions over 2 years, followed by semithin histologic analysis and transmission electron microscopy (TEM). Results:Although most drusen gradually increased in size, a portion spontaneously regressed or collapsed over 2 years. Histologic analyses showed that soft drusen exhibit hypertrophy and dysmorphia of overlying retinal pigment epithelium (RPE), as seen in early and intermediate AMD, but do not exhibit RPE atrophy, RPE migration, or photoreceptor degeneration characteristic of advanced AMD. Ultrastructure of soft drusen showed abundant lipid particles within Bruch's membrane and AMD-related basal linear deposits (BlinD) resembling those in human drusen. Conclusions:The dynamic remodeling, histologic findings, and ultrastructural features of soft drusen in aged rhesus macaques support nonhuman primates as an animal model of early AMD and reveal important insights into drusen biogenesis and AMD development.

SUBMITTER: Yiu G

PROVIDER: S-EPMC7326602 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Long-term Evolution and Remodeling of Soft Drusen in Rhesus Macaques.

Yiu Glenn G Chung Sook Hyun SH Mollhoff Iris Natalie IN Wang Yinwen Y Nguyen Uyen Tu UT Shibata Bradley B Cunefare David D Farsiu Sina S Roberts Jeffrey J Thomasy Sara M SM

Investigative ophthalmology & visual science 20200201 2

<h4>Purpose</h4>To characterize the evolution and structure of soft drusen in aged rhesus macaques using in vivo multimodal retinal imaging and ex vivo histologic and ultrastructural analyses as a nonhuman primate model of early age-related macular degeneration (AMD).<h4>Methods</h4>Multimodal imaging including fundus photography, spectral domain optical coherence tomography (SD-OCT), and fundus autofluorescence (FAF) were used to characterize and track individual drusen lesions in 20 aged rhesu ...[more]

PMID: 32084273

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Dataset Information

Long-term Evolution and Remodeling of Soft Drusen in Rhesus Macaques.

Publications

Long-term Evolution and Remodeling of Soft Drusen in Rhesus Macaques.

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