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A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode.

ABSTRACT: A uric acid (UA) electrochemical biosensor was constructed using ferrocene (Fc) decorated cuprous oxide (Cu₂O) enhanced electro-active characteristics and covalently immobilized with uricase (UOx) on glassy carbon electrode (GCE). The electrochemical characteristics of the fabricated electrode was analysed by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry (DPV). DPV studies revealed rapid response of fabricated electrode UOx/Fc/Cu₂O/GCE towards UA in a wide concentration range of 0.1-1,000 ?M with a sensitivity of 1.900 ?A mM^-1 cm^-2 and very low detection limit of 0.0596 ?M. A very low magnitude Michaelis-Menten constant (Km) value was evaluated as 34.7351 ?M which indicated the chemical attraction of the enzyme towards the UA was much higher. The developed biosensor was successfully applied to detect UA in human urine samples. Moreover, reproducibility and stability studies demonstrated the fabricated UOx/Fc/Cu₂O/GCE biosensor had high reproducibility with a RSD of 2.8% and good reusability with a RSD of 3.2%. Specificity studies results showed the fabricated biosensor had strong anti-interference ability. The improved sensor performance was attributed to the synergistic electronic properties of Cu₂O and Fc that provided enhances delectrocatalytic activity and electron transfer. The present biosensor can be extended for use in clinical settings.

SUBMITTER: Yan Q

PROVIDER: S-EPMC7327035 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode.

Yan Qinghua Q Zhi Na N Yang Li L Xu Guangri G Feng Qigao Q Zhang Qiqing Q Sun Shujuan S

Scientific reports 20200630 1

A uric acid (UA) electrochemical biosensor was constructed using ferrocene (Fc) decorated cuprous oxide (Cu<sub>2</sub>O) enhanced electro-active characteristics and covalently immobilized with uricase (UOx) on glassy carbon electrode (GCE). The electrochemical characteristics of the fabricated electrode was analysed by cyclic voltammetry, electrochemical impedance spectroscopy and differential pulse voltammetry (DPV). DPV studies revealed rapid response of fabricated electrode UOx/Fc/Cu<sub>2</ ...[more]

PMID: 32606291

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Project description:Using a simple drop-casting technique, we successfully fabricated a sensitive electrochemical sensor based on the composite of reduced graphene oxide (RGO) and multiwalled carbon nanotubes (MWCNT) deposited on the surface of a glassy carbon electrode (GCE) for individual and simultaneous measurements of hydroquinone (HQ), dopamine (DA), and uric acid (UA). The nanocomposite of RGO/MWCNT was further characterized in terms of its structural properties, surface morphology, and topography using Raman, FT-IR spectroscopy, SEM, HRTEM, and AFM. Then, the proposed sensor for simultaneous measurement of HQ, DA, and UA based on RGO/MWCNT-modified GCE was investigated for its electrochemical behavior and electroanalytical performances using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). In addition, the composition ratio between RGO and MWCT was 1 : 1 showing the highest electrochemical response for simultaneous detection of HQ, DA, and UA. Owing to the synergistic effect between RGO and MWCNT leading to excellent conductivity properties, the proposed sensor exhibited improved electrochemical response at pH 7 toward the oxidation processes of HQ, DA, and UA on the surface of modified electrode. The proposed sensor demonstrated three well-defined anodic peaks of these analytes with their linear concentrations ranges of 3.0-150.0 μM for HQ, 4.0-100.0 μM for DA, and 2.0-70.0 μM for UA. The limit of detection values for the simultaneous detection of HQ, DA, and UA were found as follows 0.400 ± 0.014, 0.500 ± 0.006, and 0.300 ± 0.016 μM, respectively. The additional features of this proposed sensor are high reproducibility and stability for the simultaneous detection of HQ, DA, and UA with negligible interference effect from interferents such as Mg2+, K+, Cl-, ascorbic acid, and glucose. An acceptable percentage of recovery was also shown by this sensor for simultaneous measurements of HQ, DA, and UA using 6 samples of human urine. In summary, the RGO/MWCNT nanocomposite has been shown to be a promising platform for rapid, simple, and reliable determination of simultaneous measurements of HQ, DA, and UA in practical applications.

Dataset Information

A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode.

Publications

A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode.

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