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GC/MS analysis of Juniperus procera extract and its activity with silver nanoparticles against Aspergillus flavus growth and aflatoxins production.

ABSTRACT: From ancient to currently, it has been hard to prevent the exposure to mycotoxigenic fungi, due to these fungi occurs naturally in the environment. This paper reports the antifungal activities of the Juniperus procera stem extract with silver nanoparticles (AgNPs) against Aspergillus flavus growth and aflatoxins production. Numerous constituents of J. procera extract were detected by GC/MS analysis. Methanolic extract at 30, 60 and 90 mg/mL inhibited the growth of A. flavus, where the inhibition reached to 50.86, 51.60 and 52.58 %. respectively while weak inhibition was observed using the aqueous extract. Growth of A. flavus was reduced using AgNPs, the highest inhibition 39.31 % was recorded at 100 ppm AgNPs. Synergistic activity was observed by applying 50 ppm of AgNPs with aqueous and methanolic extracts of J. procera . A reduction in aflatoxin B2 and G2 synthesis was observed using different concentrations of methanolic stems extract of J. procera particularly with AgNPs.

SUBMITTER: Abdelghany TM

PROVIDER: S-EPMC7327896 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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GC/MS analysis of Juniperus procera extract and its activity with silver nanoparticles against Aspergillus flavus growth and aflatoxins production.

Abdelghany T M TM Hassan Maryam M MM El-Naggar Medhat A MA

Biotechnology reports (Amsterdam, Netherlands) 20200625

From ancient to currently, it has been hard to prevent the exposure to mycotoxigenic fungi, due to these fungi occurs naturally in the environment. This paper reports the antifungal activities of the Juniperus procera stem extract with silver nanoparticles (AgNPs) against Aspergillus flavus growth and aflatoxins production. Numerous constituents of J. procera extract were detected by GC/MS analysis. Methanolic extract at 30, 60 and 90 mg/mL inhibited the growth of A. flav ...[more]

PMID: 32637346

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Project description:Aspergillus flavus produces carcinogenic and mutagenic aflatoxins, which cause economic losses and risk of food safety by contaminating grains, food and feed. In this study, we characterized two bZIP transcription factors, AflatfA and AflatfB, and their genetic interaction. Compared to the wild type (WT), AflatfA deletion and AflatfA and AflatfB double deletion both caused retarded vegetative growth of mycelia. Relative to WT, the AflatfA deletion strain (ΔAflatfA) and AflatfA and AflatfB double deletion strain (ΔAflatfAΔAflatfB) produced more sclerotia, whereas the AflatfB deletion strain (ΔAflatfB) produced less sclerotia. After 4 °C preservation and incubation at 50 °C, conidia viability dramatically decreased in the ΔAflatfA and ΔAflatfAΔAflatfB but ΔAflatfB mutants, whereas conidia viability of the ΔAflatfAΔAflatfB strain was higher after storage at 4 °C than in AflatfA mutant. Conidia of ΔAflatfA, ΔAflatfB and ΔAflatfAΔAflatfB strains significantly increased in sensitivity to H2O2 in comparison with WT. Compared to WT, the mycelium of ΔAflatfA and ΔAflatfB strains were more sensitive to H2O2; conversely, the ΔAflatfAΔAflatfB strain showed less sensitivity to H2O2. ΔAflatfA and ΔAflatfAΔAflatfB strains displayed less sensitivity to the osmotic reagents NaCl, KCl and Sorbitol, in comparison with WT and ΔAflatfB strains. When on YES medium and hosts corn and peanut, ΔAflatfA and ΔAflatfAΔAflatfB strains produced less aflatoxin B1 (AFB1) than ΔAflatfB, and the AFB1 yield of ΔAflatfB was higher than that of WT. When WT and mutants were inoculated on corn and peanut, the ΔAflatfA and ΔAflatfAΔAflatfB but not ΔAflatfB mutants produced less conidia than did WT. Taken together, this study reveals that AflatfA controls more cellular processes, and the function of AflatfA is stronger than that of AflatfB when of the same process is regulated, except the response to H2O2, which might result from the effect of AflatfA on the transcriptional level of AflatfB.

Dataset Information

GC/MS analysis of Juniperus procera extract and its activity with silver nanoparticles against Aspergillus flavus growth and aflatoxins production.

Publications

GC/MS analysis of <i>Juniperus procera</i> extract and its activity with silver nanoparticles against <i>Aspergillus flavus</i> growth and aflatoxins production.

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