Development of an LC-MS method for determination of nitrogen-containing heterocycles using mixed-mode liquid chromatography.

ABSTRACT: N-containing heterocycles (NCHs) are largely used as precursors for pharmaceuticals and can enter the environment. Some NCHs have been shown to be toxic, persistent, and very mobile in the environment. Thus, they have received increasing attention in the past years. However, the analysis of these polar compounds in environmental samples is still a challenge for liquid chromatography. This paper investigates the use of mixed-mode liquid chromatography (MMLC), which has reversed-phase and ion exchange characteristics for measurements of NCHs in water. NCHs with low pK_a (i.e., < 2.5) display mainly reversed-phase interactions (neutral species) with the stationary phase and those with higher pK_a (i.e., > 5) interact by a mixture of reversed-phase/ion exchange/HILIC mechan

SUBMITTER: Abdighahroudi MS 

PROVIDER: S-EPMC7334287 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature