Dataset Information

Isolation and characterization of organophosphorus phosphatases from Bacillus thuringiensis MB497 capable of degrading Chlorpyrifos, Triazophos and Dimethoate.

ABSTRACT: In the current investigation, bacterial strain Bacillus thuringiensis MB497 was examined for production of intracellular and extracellular organophosphorus phosphatase (OPP) enzymes. This strain produced significant amount of extracellular acidic and alkaline phosphatases. Production of neutral phosphatase was negligible. Production of OPP was generally highest at pH 11 and at 45-50 °C. However, activity and stability of OPP was highest at 37 °C and reduced at higher temperatures. OPP production was decreased after 48 h of incubation. Largely, OPP activity was inhibited by SDS and EDTA and significantly enhanced by metals (Zn++, Cu++ and Cd++). Both acidic and alkaline OPPs were capable of bio-precipitation of selected metals (Ni, Mn, Cr and Cd) up to 86-100%. When used against 50 mg/l of three OP pesticides (Chlorpyrifos, Triazophos, and Dimethoate), 81-94.6% degradation of pesticides was observed by alkaline OPP, while acidic OPP showed less degradation (61-70.5%) within 30 min of incubation.

SUBMITTER: Ambreen S

PROVIDER: S-EPMC7334429 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Isolation and characterization of organophosphorus phosphatases from <i>Bacillus thuringiensis</i> MB497 capable of degrading Chlorpyrifos, Triazophos and Dimethoate.

Ambreen Samina S Yasmin Azra A Aziz Satara S

Heliyon 20200701 7

In the current investigation, bacterial strain <i>Bacillus thuringiensis</i> MB497 was examined for production of intracellular and extracellular organophosphorus phosphatase (OPP) enzymes. This strain produced significant amount of extracellular acidic and alkaline phosphatases. Production of neutral phosphatase was negligible. Production of OPP was generally highest at pH 11 and at 45-50 °C. However, activity and stability of OPP was highest at 37 °C and reduced at higher temperatures. OPP pro ...[more]

PMID: 32642578

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Project description:We examined the role of microorganisms in the degradation of the organophosphate insecticide chlorpyrifos in soils from the United Kingdom and Australia. The kinetics of degradation in five United Kingdom soils varying in pH from 4.7 to 8.4 suggested that dissipation of chlorpyrifos was mediated by the cometabolic activities of the soil microorganisms. Repeated application of chlorpyrifos to these soils did not result in the development of a microbial population with an enhanced ability to degrade the pesticide. A robust bacterial population that utilized chlorpyrifos as a source of carbon was detected in an Australian soil. The enhanced ability to degrade chlorpyrifos in the Australian soil was successfully transferred to the five United Kingdom soils. Only soils with a pH of >/=6.7 were able to maintain this degrading ability 90 days after inoculation. Transfer and proliferation of degrading microorganisms from the Australian soil to the United Kingdom soils was monitored by molecular fingerprinting of bacterial 16S rRNA genes by PCR-denaturing gradient gel electrophoresis (DGGE). Two bands were found to be associated with enhanced degradation of chlorpyrifos. Band 1 had sequence similarity to enterics and their relatives, while band 2 had sequence similarity to strains of Pseudomonas. Liquid enrichment culture using the Australian soil as the source of the inoculum led to the isolation of a chlorpyrifos-degrading bacterium. This strain had a 16S rRNA gene with a sequence identical to that of band 1 in the DGGE profile of the Australian soil. DNA probing indicated that genes similar to known organophosphate-degrading (opd) genes were present in the United Kingdom soils. However, no DNA hybridization signal was detected for the Australian soil or the isolated degrader. This indicates that unrelated genes were present in both the Australian soil and the chlorpyrifos-degrading isolate. These results are consistent with our observations that degradation of chlorpyrifos in these systems was unusual, as it was growth linked and involved complete mineralization. As the 16S rRNA gene of the isolate matched a visible DGGE band from the Australian soil, the isolate is likely to be both prominent and involved in the degradation of chlorpyrifos in this soil.

Dataset Information

Isolation and characterization of organophosphorus phosphatases from Bacillus thuringiensis MB497 capable of degrading Chlorpyrifos, Triazophos and Dimethoate.

Publications

Isolation and characterization of organophosphorus phosphatases from <i>Bacillus thuringiensis</i> MB497 capable of degrading Chlorpyrifos, Triazophos and Dimethoate.

Similar Datasets

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