Dataset Information

Efficient Photoelectron Capture by Ni Decoration in Methanosarcina barkeri-CdS Biohybrids for Enhanced Photocatalytic CO₂-to-CH₄ Conversion.

ABSTRACT: Semi-artificial photosynthesis (biohybrid) provides an intriguing opportunity for efficient CO₂-to-CH₄ conversion. However, creating a desirable semiconductor in biohybrids remains a great challenge. Here, by doping Ni into CdS nanoparticles, we have successfully developed the Methanosarcina barkeri-Ni:CdS biohybrids. The CH₄ yield by the M. barkeri-Ni_(0.75%):CdS biohybrids was approximately 250% higher than that by the M. barkeri-CdS biohybrids. The suitable Ni dopants serve as an effective electron sink, which accelerates the photoelectron transfer in biohybrids. In addition, Ni doping changes the metabolic status of M. barkeri and results in a higher expression of a series of proteins for electron transfer, energy conversion, and CO₂ fixation. These increased proteins can promote the photoelectron capture by M. barkeri and injection into cells, which trigger a higher intracellular reduction potential to drive the reduction of CO₂ to CH₄. Our discovery will offer a promising strategy for the optimization of biohybrids in the solar-to-chemical conversion.

SUBMITTER: Ye J

PROVIDER: S-EPMC7334578 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Efficient Photoelectron Capture by Ni Decoration in Methanosarcina barkeri-CdS Biohybrids for Enhanced Photocatalytic CO2-to-CH4 Conversion.

Ye Jie J Ren Guoping G Kang Li L Zhang Yiyun Y Liu Xing X Zhou Shungui S He Zhen Z

iScience 20200620 7

Semi-artificial photosynthesis (biohybrid) provides an intriguing opportunity for efficient CO2-to-CH4 conversion. However, creating a desirable semiconductor in biohybrids remains a great challenge. Here, by doping Ni into CdS nanoparticles, we have successfully developed the Methanosarcina barkeri-Ni:CdS biohybrids. The CH4 yield by the M. barkeri-Ni(0.75%):CdS biohybrids was approximately 250% higher than that by the M. barkeri-CdS biohybrids. The s ...[more]

PMID: 32623335

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Project description:BackgroundAnaerobic digestion of easily degradable biowaste can lead to the accumulation of volatile fatty acids, which will cause environmental stress to the sensitive methanogens consequently. The metabolic characteristics of methanogens under acetate stress can affect the overall performance of mixed consortia. Nevertheless, there exist huge gaps in understanding the responses of the dominant methanogens to the stress, e.g., Methanosarcinaceae. Such methanogens are resistant to environmental deterioration and able to utilize multiple carbon sources. In this study, transcriptomic and proteomic analyses were conducted to explore the responses of Methanosarcina barkeri strain MS at different acetate concentrations of 10, 25, and 50 mM.ResultsThe trend of OD600 and the regulation of the specific genes in 50 mM acetate, indicated that high concentration of acetate promoted the acclimation of M. barkeri to acetate stress. Acetate stress hindered the regulation of quorum sensing and thereby eliminated the advantages of cell aggregation, which was beneficial to resist stress. Under acetate stress, M. barkeri allocated more resources to enhance the uptake of iron to maintain the integrities of electron-transport chains and other essential biological processes. Comparing with the initial stages of different acetate concentrations, most of the genes participating in acetoclastic methanogenesis did not show significantly different expressions except hdrB1C1, an electron-bifurcating heterodisulfide reductase participating in energy conversion and improving thermodynamic efficiency. Meanwhile, vnfDGHK and nifDHK participating in nitrogen fixation pathway were upregulated.ConclusionIn this work, transcriptomic and proteomic analyses are combined to reveal the responses of M. barkeri to acetate stress in terms of central metabolic pathways, which provides basic clues for exploring the responses of other specific methanogens under high organics load. Moreover, the results can also be used to gain insights into the complex interactions and geochemical cycles among natural or engineered populations. Furthermore, these findings also provide the potential for designing effective and robust anaerobic digesters with high organic loads.

Dataset Information

Efficient Photoelectron Capture by Ni Decoration in Methanosarcina barkeri-CdS Biohybrids for Enhanced Photocatalytic CO₂-to-CH₄ Conversion.

Publications

Efficient Photoelectron Capture by Ni Decoration in Methanosarcina barkeri-CdS Biohybrids for Enhanced Photocatalytic CO<sub>2</sub>-to-CH<sub>4</sub> Conversion.

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