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Structural characterization of borneol dehydrogenase from Pseudomonas sp. TCU-HL1.

ABSTRACT: During the microbial degradation of borneol, a bicyclic plant monoterpene, it is first converted into camphor by borneol dehydrogenase (BDH) and then enters a known camphor-degradation pathway. Previously, a recombinant Pseudomonas BDH was found in inclusion bodies when expressed in Escherichia coli. After refolding, it was still unstable and was difficult to concentrate. Here, the protein-expression conditions were improved by changing the medium from lysogeny broth to Terrific Broth, yielding a soluble form of the enzyme with higher activity. The protein was crystallized and its 3D structure was determined by X-ray diffraction. Like other known homologues such as quinuclidinone reductase, the protein forms a tetramer with subunits containing Rossmann folds. Structural comparison revealed major differences in the C-terminal helices and the associated loops. It is likely that these regions contain the determinants for substrate recognition.

SUBMITTER: Khine AA

PROVIDER: S-EPMC7336358 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Structural characterization of borneol dehydrogenase from Pseudomonas sp. TCU-HL1.

Khine Aye Aye AA Chen Hao Ping HP Huang Kai Fa KF Ko Tzu Ping TP

Acta crystallographica. Section F, Structural biology communications 20200701 Pt 7

During the microbial degradation of borneol, a bicyclic plant monoterpene, it is first converted into camphor by borneol dehydrogenase (BDH) and then enters a known camphor-degradation pathway. Previously, a recombinant Pseudomonas BDH was found in inclusion bodies when expressed in Escherichia coli. After refolding, it was still unstable and was difficult to concentrate. Here, the protein-expression conditions were improved by changing the medium from lysogeny broth to Terrific Broth, yielding ...[more]

PMID: 32627746

Similar Datasets

Borneol Dehydrogenase from Pseudomonas sp. Strain TCU-HL1 Catalyzes the Oxidation of (+)-Borneol and Its Isomers to Camphor.

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