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Translation at first sight: the influence of leading codons.

ABSTRACT: First triplets of mRNA coding region affect the yield of translation. We have applied the flowseq method to analyze >30 000 variants of the codons 2-11 of the fluorescent protein reporter to identify factors affecting the protein synthesis. While the negative influence of mRNA secondary structure on translation has been confirmed, a positive role of rare codons at the beginning of a coding sequence for gene expression has not been observed. The identity of triplets proximal to the start codon contributes more to the protein yield then more distant ones. Additional in-frame start codons enhance translation, while Shine-Dalgarno-like motifs downstream the initiation codon are inhibitory. The metabolic cost of amino acids affects the yield of protein in the poor medium. The most efficient translation was observed for variants with features resembling those of native Escherichia coli genes.

SUBMITTER: Osterman IA

PROVIDER: S-EPMC7337518 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Translation at first sight: the influence of leading codons.

Osterman Ilya A IA Chervontseva Zoe S ZS Evfratov Sergey A SA Sorokina Alena V AV Rodin Vladimir A VA Rubtsova Maria P MP Komarova Ekaterina S ES Zatsepin Timofei S TS Kabilov Marsel R MR Bogdanov Alexey A AA Gelfand Mikhail S MS Dontsova Olga A OA Sergiev Petr V PV

Nucleic acids research 20200701 12

First triplets of mRNA coding region affect the yield of translation. We have applied the flowseq method to analyze >30 000 variants of the codons 2-11 of the fluorescent protein reporter to identify factors affecting the protein synthesis. While the negative influence of mRNA secondary structure on translation has been confirmed, a positive role of rare codons at the beginning of a coding sequence for gene expression has not been observed. The identity of triplets proximal to the start codon co ...[more]

PMID: 32427319

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Translation at first sight: the influence of leading codons.

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Translation at first sight: the influence of leading codons.

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