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The Legionella Effector Kinase LegK7 Hijacks the Host Hippo Pathway to Promote Infection.


ABSTRACT: The intracellular pathogen Legionella pneumophila encodes translocated effector proteins that modify host cell processes to support bacterial survival and growth. Here, we show that the L. pneumophila effector protein LegK7 hijacks the conserved Hippo signaling pathway by molecularly mimicking host Hippo kinase (MST1 in mammals), which is the key regulator of pathway activation. LegK7, like Hippo/MST1, phosphorylates the scaffolding protein MOB1, which triggers a signaling cascade resulting in the degradation of the transcriptional regulators TAZ and YAP1. Transcriptome analysis revealed that LegK7-mediated targeting of TAZ and YAP1 alters the transcriptional profile of mammalian macrophages, a key cellular target of L. pneumophila infection. Specifically, genes targeted by the transcription factor PPAR?, which is regulated by TAZ, displayed altered expression, and continuous interference with PPAR? activity rendered macrophages less permissive to L. pneumophila intracellular growth. Thus, a conserved L. pneumophila effector kinase exploits the Hippo pathway to promote bacterial growth and infection.

SUBMITTER: Lee PC 

PROVIDER: S-EPMC7343393 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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The Legionella Effector Kinase LegK7 Hijacks the Host Hippo Pathway to Promote Infection.

Lee Pei-Chung PC   Machner Matthias P MP  

Cell host & microbe 20180901 3


The intracellular pathogen Legionella pneumophila encodes translocated effector proteins that modify host cell processes to support bacterial survival and growth. Here, we show that the L. pneumophila effector protein LegK7 hijacks the conserved Hippo signaling pathway by molecularly mimicking host Hippo kinase (MST1 in mammals), which is the key regulator of pathway activation. LegK7, like Hippo/MST1, phosphorylates the scaffolding protein MOB1, which triggers a signaling cascade resulting in t  ...[more]

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