Development and Validation of an in-House Library of Colombian Candida auris Strains with MALDI-TOF MS to Improve Yeast Identification.

ABSTRACT: : Background: Candida auris is characterized for having a high genetic variability among species. MALDI-TOF MS library contains spectra from only three strains of C. auris, which makes difficult the identification process and gives low scores at the species level. Our aim was to construct and validate an internal library to improve C. auris identification with Colombian clinical strains.

Methods

From 30 clinical strains, 770 mass spectra were obtained for the construction of the database. The validation was performed with 300 strains to compare the identification results in the BDAL and C. auris Colombia libraries.

Results

Our library allowed a complete, 100% identification of the evaluated strains and a significant improvement in the scores

SUBMITTER: Ceballos-Garzon A 

PROVIDER: S-EPMC7344545 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature