The genome polishing tool POLCA makes fast and accurate corrections in genome assemblies.

ABSTRACT: The introduction of third-generation DNA sequencing technologies in recent years has allowed scientists to generate dramatically longer sequence reads, which when used in whole-genome sequencing projects have yielded better repeat resolution and far more contiguous genome assemblies. While the promise of better contiguity has held true, the relatively high error rate of long reads, averaging 8-15%, has made it challenging to generate a highly accurate final sequence. Current long-read sequencing technologies display a tendency toward systematic errors, in particular in homopolymer regions, which present additional challenges. A cost-effective strategy to generate highly contiguous assemblies with a very low overall error rate is to combine long reads with low-cost short-read data, which cu

SUBMITTER: Zimin AV 

PROVIDER: S-EPMC7347232 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature