Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing.
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ABSTRACT:
SUBMITTER: Park H
PROVIDER: S-EPMC7349485 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing.
Park Hanseul H Shin Jaein J Choi Hwan H Cho Byounggook B Kim Jongpil J
Cells 20200610 6
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos ...[more]
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