Ontology highlight
ABSTRACT:
SUBMITTER: Gomez J
PROVIDER: S-EPMC7351060 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Gómez Juan J Melón Santiago S Boga José A JA Alvarez-Argüelles Marta E ME Rojo-Alba Susana S Leal-Negredo Alvaro A Castello-Abietar Cristian C Alvarez Victoria V Cuesta-Llavona Elías E Coto Eliecer E
Journal of virological methods 20200710
Due to the huge demand for SARS-Cov-2 determination,alternatives to the standard qtPCRtestsare potentially useful for increasing the number of samples screened. Our aim was to develop a direct fluorescent PCR capillary-electrophoresis detection of the viral genome. We validated this approach on several SARS-Cov-2 positive and negative samples.We isolated the naso-pharingealRNA from 20 positive and 10 negative samples. The cDNA was synthesised and two fragments of the SARS-Cov-2 were amplified. O ...[more]