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Computer-assisted beat-pattern analysis and the flagellar waveforms of bovine spermatozoa.


ABSTRACT: Obstructed by hurdles in information extraction, handling and processing, computer-assisted sperm analysis systems have typically not considered in detail the complex flagellar waveforms of spermatozoa, despite their defining role in cell motility. Recent developments in imaging techniques and data processing have produced significantly improved methods of waveform digitization. Here, we use these improvements to demonstrate that near-complete flagellar capture is realizable on the scale of hundreds of cells, and, further, that meaningful statistical comparisons of flagellar waveforms may be readily performed with widely available tools. Representing the advent of high-fidelity computer-assisted beat-pattern analysis, we show how such a statistical approach can distinguish between samples using complex flagellar beating patterns rather than crude summary statistics. Dimensionality-reduction techniques applied to entire samples also reveal qualitatively distinct components of the beat, and a novel data-driven methodology for the generation of representative synthetic waveform data is proposed.

SUBMITTER: Walker BJ 

PROVIDER: S-EPMC7353979 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Computer-assisted beat-pattern analysis and the flagellar waveforms of bovine spermatozoa.

Walker Benjamin J BJ   Phuyal Shiva S   Ishimoto Kenta K   Tung Chih-Kuan CK   Gaffney Eamonn A EA  

Royal Society open science 20200617 6


Obstructed by hurdles in information extraction, handling and processing, computer-assisted sperm analysis systems have typically not considered in detail the complex flagellar waveforms of spermatozoa, despite their defining role in cell motility. Recent developments in imaging techniques and data processing have produced significantly improved methods of waveform digitization. Here, we use these improvements to demonstrate that near-complete flagellar capture is realizable on the scale of hund  ...[more]

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