Three types of HLA-G+ extravillous trophoblasts that have distinct immune regulatory properties.

ABSTRACT: During pregnancy, invading HLA-G+ extravillous trophoblasts (EVT) play a key role in placental development, uterine spiral artery remodeling, and prevention of detrimental maternal immune responses to placental and fetal antigens. Failures of these processes are suggested to play a role in the development of pregnancy complications, but very little is known about the underlying mechanisms. Here we present validated methods to purify and culture primary HLA-G+ EVT from the placental disk and chorionic membrane from healthy term pregnancy. Characterization of HLA-G+ EVT from term pregnancy compared to first trimester revealed their unique phenotypes, gene expression profiles, and differing capacities to increase regulatory T cells (Treg) during coculture assays, features that cannot be captu

SUBMITTER: Papuchova H 

PROVIDER: S-EPMC7355041 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature