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Apparent lack of spill-over of parasites from an invasive anuran: PCR detects Entamoeba in cane toads (Rhinella marina) but not in sympatric Australian native frogs.

ABSTRACT: The recent detection of a novel amoebozoan parasite (Entamoeba sp. CT1) killing invasive cane toads (Rhinella marina) in tropical Australia raises concerns of potential spill-over into native anuran populations. Considering the vulnerability of anuran communities globally, Entamoeba sp. CT1 may pose a serious threat to anuran biodiversity. Through PCR-based detection and molecular identification, we investigated the prevalence of Entamoeba spp. in the faeces and colon tissue of cane toads (Rhinella marina) and eleven native Australian frog species from a single locality in the Northern Territory. No Entamoeba DNA was detected in samples of native frog faeces (N = 57) or colons (N = 17). Entamoeba DNA was detected in 24% of 45 cane toads (95%CI 14.08-38.82). Both E. ranarum and Entamoeba sp. CT1 were present in cane toads. The failure of faecal samples to indicate Entamoeba spp. in infected cane toads may be due to cysts in faeces being shed intermittently, degraded before analysis, or impervious to lysis prior to DNA isolation. Our results suggest that native frogs do not carry the pathogen in an area where 20-30% of cane toads are infected with Entamoeba sp. CT1. We demonstrate the importance of recognising PCR inhibition prior to molecular diagnostics, and the apparent inadequacy of faecal samples for the detection of Entamoeba spp. in anurans.

SUBMITTER: Rivory P

PROVIDER: S-EPMC7355385 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Apparent lack of spill-over of parasites from an invasive anuran: PCR detects Entamoeba in cane toads (Rhinella marina) but not in sympatric Australian native frogs.

Rivory Phoebe P Brown Gregory G Shilton Cathy C Shine Richard R Šlapeta Jan J

International journal for parasitology. Parasites and wildlife 20200630

The recent detection of a novel amoebozoan parasite (Entamoeba sp. CT1) killing invasive cane toads (Rhinella marina) in tropical Australia raises concerns of potential spill-over into native anuran populations. Considering the vulnerability of anuran communities globally, Entamoeba sp. CT1 may pose a serious threat to anuran biodiversity. Through PCR-based detection and molecular identification, we investigated the prevalence of Entamoeba spp. in the faeces and colon ...[more]

PMID: 32685367

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Project description:Invasive species must deal with novel challenges, both from the alien environment and from pressures arising from range expansion per se (e.g. spatial sorting). Those conditions can create geographical variation in behaviour across the invaded range, as has been documented across regions of Australia invaded by cane toads; range-edge toads are more exploratory and willing to take risks than are conspecifics from the range-core. That behavioural divergence might be a response to range expansion and invasion per se, or to the different environments encountered. Climate differs across the cane toads' invasion range from the wet tropics of Queensland to the seasonally dry climates of northwestern Western Australia. The different thermal and hydric regimes may affect behavioural traits via phenotypic plasticity or through natural selection. We cannot tease apart the effects of range expansion versus climate in an expanding population but can do so in a site where the colonizing species was simultaneously released in all suitable areas, thus removing any subsequent phase of range expansion. Cane toads were introduced to Hawai'i in 1932; and thence to Australia in 1935. Toads were released in all major sugarcane-growing areas in Hawai'i within a 12-month period. Hence, Hawai'ian cane toads provide an opportunity to examine geographical divergence in behavioural traits in a climatically diverse region (each island has both wet and dry sides) in the absence of range expansion subsequent to release. We conducted laboratory-based behavioural trials testing exploration, risk-taking and response to novelty using field-caught toads from the wet and dry sides of two Hawai'ian islands (Oahu and Hawai'i). Toads from the dry side of Oahu had a higher propensity to take risks than did toads from the dry side of Hawai'i. Toads from Oahu were also more exploratory than were conspecifics from the island of Hawai'i. However, toads from wet versus dry climates were similar in all behaviours that we scored, suggesting that founder effects, genetic drift, or developmentally plastic responses to ecological factors other than climate may have driven behavioural divergence between islands.

Dataset Information

Apparent lack of spill-over of parasites from an invasive anuran: PCR detects Entamoeba in cane toads (Rhinella marina) but not in sympatric Australian native frogs.

Publications

Apparent lack of spill-over of parasites from an invasive anuran: PCR detects <i>Entamoeba</i> in cane toads (<i>Rhinella marina</i>) but not in sympatric Australian native frogs.

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