Dataset Information

Salicylic Acid Stimulates Antioxidant Defense and Osmolyte Metabolism to Alleviate Oxidative Stress in Watermelons under Excess Boron.

ABSTRACT: Boron (B) is a microelement required in vascular plants at a high concentration that produces excess boron and toxicity in many crops. B stress occurs widely and limits plant growth and crop productivity worldwide. Salicylic acid (SA) is an essential hormone in plants and is a phenolic compound. The goal of this work is to explore the role of SA in the alleviation of excess B (10 mg L-1) in watermelon plants at a morphological and biochemical level. Excess boron altered the nutrient concentrations and caused a significant reduction in morphological criteria; chlorophyll a, b, and carotenoids; net photosynthetic rate; and the stomatal conductance and transpiration rate of watermelon seedlings, while intercellular carbon dioxide (CO2) was significantly increased compared to the control plants (0.5 mg L-1 B). Furthermore, excess boron accelerated the generation of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) and induced cellular oxidative injury. The application of exogenous SA significantly increased chlorophyll and carotenoid contents in plants exposed to excess B (10 mg L-1), in line with the role of SA in alleviating chlorosis caused by B stress. Exogenously applied SA promoted photosynthesis and, consequently, biomass production in watermelon seedlings treated with a high level of B (10 mg L-1) by reducing B accumulation, lipid peroxidation, and the generation of H2O2, while significantly increasing levels of the most reactive ROS, OH-. SA also activated antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) and protected the seedlings from an ROS induced cellular burst. In conclusion, SA can be used to alleviate the adverse effects of excess boron.

SUBMITTER: Moustafa-Farag M

PROVIDER: S-EPMC7357100 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Salicylic Acid Stimulates Antioxidant Defense and Osmolyte Metabolism to Alleviate Oxidative Stress in Watermelons under Excess Boron.

Moustafa-Farag Mohamed M Mohamed Heba I HI Mahmoud Ahmed A Elkelish Amr A Misra Amarendra N AN Guy Kateta Malangisha KM Kamran Muhammad M Ai Shaoying S Zhang Mingfang M

Plants (Basel, Switzerland) 20200608 6

Boron (B) is a microelement required in vascular plants at a high concentration that produces excess boron and toxicity in many crops. B stress occurs widely and limits plant growth and crop productivity worldwide. Salicylic acid (SA) is an essential hormone in plants and is a phenolic compound. The goal of this work is to explore the role of SA in the alleviation of excess B (10 mg L<sup>-1</sup>) in watermelon plants at a morphological and biochemical level. Excess boron altered the nutrient c ...[more]

PMID: 32521755

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Project description:BackgroundSnakebites remain a major life-threatening event worldwide. It is still difficult to make a positive identification of snake species by clinicians in both Western medicine and Chinese medicine. The main reason for this is a shortage of diagnostic biomarkers and lack of knowledge about pathways of venom-induced toxicity. In traditional Chinese medicine, snakebites are considered to be treated with wind, fire, and wind-fire toxin, but additional studies are required.MethodsCases of snakebite seen at the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine were grouped as follows: fire toxin - including four cases of bites by Agkistrodon acutus and three bites by Trimeresurus stejnegeri - and wind-fire toxin - four cases of bites by vipers and three bites by cobras. Serum protein quantification was performed using LC-MS/MS. Differential abundance proteins (DAPs) were identified from comparison of snakebites of each snake species and healthy controls. The protein interaction network was constructed using STITCH database.ResultsPrincipal component analysis and hierarchical clustering of 474 unique proteins exhibited protein expression profiles of wind-fire toxins that are distinct from that of fire toxins. Ninety-three DAPs were identified in each snakebite subgroup as compared with healthy control, of which 38 proteins were found to have significantly different expression levels and 55 proteins displayed no expression in one subgroup, by subgroup comparison. GO analysis revealed that the DAPs participated in bicarbonate/oxygen transport and hydrogen peroxide catabolic process, and affected carbon-oxygen lyase activity and heme binding. Thirty DAPs directly or indirectly acted on hydrogen peroxide in the interaction network of proteins and drug compounds. The network was clustered into four groups: lipid metabolism and transport; IGF-mediated growth; oxygen transport; and innate immunity.ConclusionsOur results show that the pathways of snake venom-induced toxicity may form a protein network of antioxidant defense by regulating oxidative stress through interaction with hydrogen peroxide.

Dataset Information

Salicylic Acid Stimulates Antioxidant Defense and Osmolyte Metabolism to Alleviate Oxidative Stress in Watermelons under Excess Boron.

Publications

Salicylic Acid Stimulates Antioxidant Defense and Osmolyte Metabolism to Alleviate Oxidative Stress in Watermelons under Excess Boron.

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