Dataset Information

Biomass addition alters community assembly in ultrafiltration membrane biofilms.

ABSTRACT: Freshwater biofilms assemble from a pool of rare water column genotypes. Random density fluctuations and temporal species turnover of functionally equivalent potential colonizers result in compositional variability of newly formed biofilm communities. We hypothesized that stronger environmental filtering as induced by enhanced substrate levels might reduce the impact of a locally variable pool of colonizers and instead select for more universal habitat specialists. Our model were heterotrophic biofilms that form on membranes during gravity-driven ultrafiltration of lake water. In four separate experiments, biomass of the cyanobacterium Microcystis was added to the feed water of one set of treatments (BM) and the resulting biofilm communities were compared to unamended controls (CTRL). Biomass addition led to a significant shift of community assembly processes: Replicate BM biofilms were more similar to each other than by chance in 3 of 4 experiments, whereas the opposite was the case for CTRL communities. Moreover, BM communities were more stochastically assembled across experiments from a common 'regional' pool of biofilm colonizers, whereas the composition of CTRL communities was mainly determined by experiment-specific 'local' genotypes. Interestingly, community assembly processes were also related to both, physiology (aerobic vs. anaerobic lifestyle) and the phylogenetic affiliation of biofilm bacteria.

SUBMITTER: Silva MOD

PROVIDER: S-EPMC7360762 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Biomass addition alters community assembly in ultrafiltration membrane biofilms.

Silva Marisa O D MOD Pernthaler Jakob J

Scientific reports 20200714 1

Freshwater biofilms assemble from a pool of rare water column genotypes. Random density fluctuations and temporal species turnover of functionally equivalent potential colonizers result in compositional variability of newly formed biofilm communities. We hypothesized that stronger environmental filtering as induced by enhanced substrate levels might reduce the impact of a locally variable pool of colonizers and instead select for more universal habitat specialists. Our model were heterotrophic b ...[more]

PMID: 32665605

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Project description:The presence of more species in the community of a sampling site (α diversity) typically increases ecosystem functions via nonrandom processes like resource partitioning. When considering multiple communities, we hypothesize that higher compositional difference (β diversity) increases overall functions of these communities. Further, we hypothesize that the β diversity effect is more positive when β diversity is increased by nonrandom assembly processes. To test these hypotheses, we collected bacterioplankton along a transect of 6 sampling sites in the southern East China Sea in 14 cruises. For any pairs of the 6 sites within a cruise, we calculated the Bray-Curtis index to represent β diversity and summed bacterial biomass as a proxy to indicate the overall function of the two communities. We then calculated deviation of observed mean pairwise phylogenetic similarities among species in two communities from random to represent the influences of nonrandom processes. The bacterial β diversity was found to positively affect the summed bacterial biomass; however, the effect varied among cruises. Cross-cruise comparison indicated that the β diversity effect increased with the nonrandom processes selecting for phylogenetically dissimilar species. This study extends biodiversity-ecosystem functioning research to the scale of multiple sites and enriches the framework by considering community assembly processes. IMPORTANCE The implications of our analyses are twofold. First, we emphasize the importance of studying β diversity. We expanded the current biodiversity-ecosystem functioning framework from single to multiple sampling sites and investigated the influences of species compositional differences among sites on the overall functioning of these sites. Since natural ecological communities never exist alone, our analyses allow us to more holistically perceive the role of biodiversity in natural ecosystems. Second, we took community assembly processes into account to attain a more mechanistic understanding of the impacts of biodiversity on ecosystem functioning.

Project description:IntroductionTajikistan is a typical mountainous country covered by different mountain grasslands that are important pasture resources. Recently, grassland degradation has become widespread due to climate change and human activities and fertilization has been used to improve grassland production. However, fertilizer inputs can substantially alter species diversity, but it is uncl\ear how productivity and species diversity respond to nutrient enrichment in the mountain meadows of Tajikistan.MethodsTherefore, a 5-year (2018-2022) continuous in-situ mineral fertilizer experiment was conducted to examine the effects of three nitrogen (N) levels (0, 30, and 90 kg N ha-1 year-1), two phosphorus (P) levels (0 and 30 kg P ha-1 year-1), and their combinations on above-ground biomass (AGB) and species diversity in a mountain meadow grassland in Ziddi, Varzob region, Tajikistan. Five species diversity metrics-Margalef's species richness (Dma), the Shannon-Wiener index (H), the Simpson index (C), Pielou's equitability index (Epi), and the Evar Species Evenness index (Evar)-were used to measure species diversity.Results and discussionsThe results indicated that the addition of different N and P amounts and their various combinations considerably increased both total and dominant species AGB, with the highest increase occurring in the N90P30 (90 kg N ha-1 year-1 combined with 30 kg P ha-1 year-1) treatment in 2022; during the experiment, the importance value of Prangos pabularia (dominant species) first decreased and then increased, but its dominant status did not change or fluctuate among the years. Furthermore, N, P, and their different combinations had no significant effect on species diversity (Dma, H, C, Epi, and Evar). All the species diversity indexes fluctuated among years, but there was no interaction with mineral fertilizer addition. Total AGB had a negative relationship with species diversity and low concentration N fertilizer addition (N30; P30) strengthened this negative trend. However, this trend decreased under the high N fertilizer condition (N90P30). Overall, nutrient addition to the natural mountain grassland of the Varzob region improved AGB, which meant that there was more forage for local animals. Mineral fertilizers had no significant effect on species diversity, but may enhance P. pabularia dominance in the future, which will help maintain the stability of the plant community and improve the quality of the forage because P. pabularia is an excellent and important winter fodder. Our study suggests that scientific nutrient management could effectively promote grassland production, conserve plant variety, and regenerate degraded grassland, which will counteract the desertification process in northwest Tajikistan mountain meadows.

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Biomass addition alters community assembly in ultrafiltration membrane biofilms.

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Biomass addition alters community assembly in ultrafiltration membrane biofilms.

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