Dataset Information

Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5? Binding to the Viral Core.

ABSTRACT: Disruption of cyclophilin A (CypA)-capsid interactions affects HIV-1 replication in human lymphocytes. To understand this mechanism, we utilize human Jurkat cells, peripheral blood mononuclear cells (PBMCs), and CD4+ T cells. Our results show that inhibition of HIV-1 infection caused by disrupting CypA-capsid interactions is dependent on human tripartite motif 5? (TRIM5?hu), showing that TRIM5?hu restricts HIV-1 in CD4+ T cells. Accordingly, depletion of TRIM5?hu in CD4+ T cells rescues HIV-1 that fail to interact with CypA, such as HIV-1-P90A. We found that TRIM5?hu binds to the HIV-1 core. Disruption of CypA-capsid interactions fail to affect HIV-1-A92E/G94D infection, correlating with the loss of TRIM5?hu binding to HIV-1-A92E/G94D cores. Disruption of CypA-capsid interactions in primary cells has a greater inhibitory effect on HIV-1 when compared to Jurkat cells. Consistent with TRIM5? restriction, disruption of CypA-capsid interactions in CD4+ T cells inhibits reverse transcription. Overall, our results reveal that CypA binding to the core protects HIV-1 from TRIM5?hu restriction.

SUBMITTER: Selyutina A

PROVIDER: S-EPMC7363000 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5α Binding to the Viral Core.

Selyutina Anastasia A Persaud Mirjana M Simons Lacy M LM Bulnes-Ramos Angel A Buffone Cindy C Martinez-Lopez Alicia A Scoca Viviana V Di Nunzio Francesca F Hiatt Joseph J Marson Alexander A Krogan Nevan J NJ Hultquist Judd F JF Diaz-Griffero Felipe F

Cell reports 20200301 11

Disruption of cyclophilin A (CypA)-capsid interactions affects HIV-1 replication in human lymphocytes. To understand this mechanism, we utilize human Jurkat cells, peripheral blood mononuclear cells (PBMCs), and CD4+ T cells. Our results show that inhibition of HIV-1 infection caused by disrupting CypA-capsid interactions is dependent on human tripartite motif 5α (TRIM5αhu), showing that TRIM5αhu restricts HIV-1 in CD4+ T cells. Accordingly, depletion ...[more]

PMID: 32187548

Similar Datasets

Project description:BackgroundHIV-1 is inhibited early after entry into cells expressing some simian orthologues of the tripartite motif protein family member TRIM5?. Mutants of the human orthologue (TRIM5?hu) can also provide protection against HIV-1. The host protein cyclophilin A (CypA) binds incoming HIV-1 capsid (CA) proteins and enhances early stages of HIV-1 replication by unknown mechanisms. On the other hand, the CA-CypA interaction is known to increase HIV-1 susceptibility to restriction by TRIM5?. Previously, the mutation V86M in the CypA-binding loop of HIV-1 CA was found to be selected upon serial passaging of HIV-1 in cells expressing Rhesus macaque TRIM5? (TRIM5?rh). The objectives of this study were (i) to analyze whether V86M CA allows HIV-1 to escape mutants of TRIM5?hu, and (ii) to characterize the role of CypA in the resistance to TRIM5? conferred by V86M.ResultsWe find that in single-cycle HIV-1 vector transduction experiments, V86M confers partial resistance against R332G-R335G TRIM5?hu and other TRIM5?hu variable 1 region mutants previously isolated in mutagenic screens. However, V86M HIV-1 does not seem to be resistant to R332G-R335G TRIM5?hu in a spreading infection context. Strikingly, restriction of V86M HIV-1 vectors by TRIM5?hu mutants is mostly insensitive to the presence of CypA in infected cells. NMR experiments reveal that V86M alters CypA interactions with, and isomerisation of CA. On the other hand, V86M does not affect the CypA-mediated enhancement of HIV-1 replication in permissive human cells. Finally, qPCR experiments show that V86M increases HIV-1 transport to the nucleus of cells expressing restrictive TRIM5?.ConclusionsOur study shows that V86M de-couples the two functions associated with CA-CypA binding, i.e. the enhancement of restriction by TRIM5? and the enhancement of HIV-1 replication in permissive human cells. V86M enhances the early stages of HIV-1 replication in restrictive cells by improving nuclear import. In summary, our data suggest that HIV-1 escapes restriction by TRIM5? through the selective disruption of CypA-dependent, TRIM5?-mediated inhibition of nuclear import. However, V86M does not seem to relieve restriction of a spreading HIV-1 infection by TRIM5?hu mutants, underscoring context-specific restriction mechanisms.

Dataset Information

Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5? Binding to the Viral Core.

Publications

Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5α Binding to the Viral Core.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure