Dataset Information

Draft Genome Sequence of Fusarium oxysporum f. sp. albedinis Strain Foa 133, the Causal Agent of Bayoud Disease on Date Palm.

ABSTRACT: Fusarium oxysporum f. sp. albedinis is the causal agent of vascular wilt of date palm. Here, we report the genome assembly of the Foa 133 strain, which consists of 3,325 contigs with a total length of 56,228,901?bp, a GC content of 47.42%, an N 50 value of 131,587?bp, and 3,684 predicted genes.

SUBMITTER: Khayi S

PROVIDER: S-EPMC7365794 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Fusarium oxysporum f. sp. albedinis Strain Foa 133, the Causal Agent of Bayoud Disease on Date Palm.

Khayi Slimane S Khoulassa Siham S Gaboun Fatima F Abdelwahd Rabha R Diria Ghizlane G Labhilili Mustapha M Iraqi Driss D El Guilli Mohammed M Fokar Mohamed M Mentag Rachid R

Microbiology resource announcements 20200716 29

Fusarium oxysporum f. sp. albedinis is the causal agent of vascular wilt of date palm. Here, we report the genome assembly of the Foa 133 strain, which consists of 3,325 contigs with a total length of 56,228,901 bp, a GC content of 47.42%, an N 50 value of 131,587 bp, and 3,684 predicted genes. ...[more]

PMID: 32675183

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Project description:Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc), is the most lethal soil-borne fungal pathogen infecting bananas. Foc race 1 (R1) and 4 (R4) are the two most predominant races affecting the economically important Cavendish group of bananas in India. A total of seven vegetative compatibility groups (VCGs) from three pathogenic races were isolated during our field survey and were found to be highly virulent towards cv. Grande Naine. According to comparative genome analyses, these Indian Foc VCGs were diverse in genomic organization and effector gene profiles. As a result, false-positive results were obtained with currently available molecular markers. In this context, the study has been initiated to develop PCR-based molecular markers for the unambiguous identification of Indian Foc R1 and R4 isolates. Whole-genome sequences of Foc R1 (GCA_011316005.3), Foc TR4 (GCA_014282265.3), and Foc STR4 (GCA_016802205.1), as well as the reference genomes of Foc (ASM799451v1) and F. oxysporum f. sp. lycopersici (Fol; ASM14995v2), were aligned to identify unique variable regions among the Foc races. Using putative chromosome and predicted gene comparison, race-specific unique Foc virulence genes were identified. The putative lineage-specific identified genes encoding products secreted in xylem (SIX) that may be necessary for disease development in the banana. An in silico analysis was performed and primers were designed from a region where sequences were dissimilar with other races to develop a specific marker for Foc R1, R4, TR4, and STR4. These race-specific markers allowed target amplification in the characterized highly virulent Foc isolates, and did not show any cross-amplification to any other Foc races, VCGs or banana pathogens, Fusarium species, and non-pathogenic Fusarium oxysporum isolates. The study demonstrated that the molecular markers developed for all the three Foc races of India could detect the pathogen in planta and up to 0.025 pg µL-1 DNA levels. Thus, the markers developed in this study are novel and could potentially be useful for the accurate diagnosis and detection of the Indian Foc races which are important for the effective management of the disease.

Dataset Information

Draft Genome Sequence of Fusarium oxysporum f. sp. albedinis Strain Foa 133, the Causal Agent of Bayoud Disease on Date Palm.

Publications

Draft Genome Sequence of Fusarium oxysporum f. sp. <i>albedinis</i> Strain Foa 133, the Causal Agent of Bayoud Disease on Date Palm.

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