Complete Genome Sequence of a Tomato Brown Rugose Fruit Virus Isolated in the United States.
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ABSTRACT:
SUBMITTER: Chanda B
PROVIDER: S-EPMC7365798 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Publications
Complete Genome Sequence of a <i>Tomato Brown Rugose Fruit Virus</i> Isolated in the United States.
Chanda Bidisha B Rivera Yazmín Y Nunziata Schyler O SO Galvez Marco E ME Gilliard Andrea A Ling Kai-Shu KS
Microbiology resource announcements 20200716 29
The complete genome sequence of a U.S. isolate of a <i>Tomato brown rugose fruit virus</i> (ToBRFV) (CA18-01) was obtained through Illumina and MinION sequencing. The U.S. ToBRFV isolate shared a high nucleic acid sequence identity (>99%) with known ToBRFV isolates. Phylogenetic analysis revealed a tight cluster for ToBRFV isolates throughout the world, suggesting a short evolutionary history. ...[more]
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Project description:The tobamovirus tomato brown rugose fruit virus (ToBRFV), a major threat to tomato production worldwide, has recently been documented in mixed infections with the potexvirus pepino mosaic virus (PepMV) CH2 strain in traded tomatoes in Israel. A study of greenhouse tomato plants in Israel revealed severe new viral disease symptoms including open unripe fruits and yellow patched leaves. PepMV was only detected in mixed infections with ToBRFV in all 104 tested sites, using serological and molecular analyses. Six PepMV isolates were identified, all had predicted amino acids characteristic of CH2 mild strains excluding an isoleucine at amino acid position 995 of the replicase. High-throughput sequencing of viral RNA extracted from four selected symptomatic plants showed solely the ToBRFV and PepMV, with total aligned read ratios of 40.61% and 11.73%, respectively, indicating prevalence of the viruses. Analyses of interactions between the co-infecting viruses by sequential and mixed viral inoculations of tomato plants, at various temperatures, showed a prominent increase in PepMV titers in ToBRFV pre-inoculated plants and in mixed-infected plants at 18-25 °C, compared to PepMV-single inoculations, as analyzed by Western blot and quantitative RT-PCR tests. These results suggest that Israeli mild PepMV isolate infections, preceded by ToBRFV, could induce symptoms characteristic of PepMV aggressive strains.
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| S-EPMC10611295 | biostudies-literature
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Project description: Not available
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