Dataset Information

Clinical and histological significance of urinary CD11c⁺ macrophages in lupus nephritis.

ABSTRACT:

Background

Infiltration of immune cells into the kidney is one of the key features of lupus nephritis (LN). The presence of immune cells in the urine may be used as a non-invasive biomarker of LN. Here, we aimed to analyze the clinicopathologic significance of urinary CD11c⁺ macrophages in patients with LN.

Methods

The numbers and proportions of CD11c⁺ macrophages in the urine samples of patients with LN at the time of kidney biopsy were examined using flow cytometry. We also examined the association between the levels of urinary CD11c⁺ macrophages and the clinical and pathologic features of patients with LN.

Results

Compared with patients without LN or those with non-proliferative LN, patients with proliferative LN had significantly higher numbers and proportions of urinary CD11c⁺ macrophages, which were strongly correlated with the serum anti-dsDNA antibody titer. The numbers and proportions of urinary CD11c⁺ macrophages were significantly associated with the values of chronicity indices such as tubular atrophy and interstitial fibrosis. No significant relationships were found between the levels of urinary CD11c⁺ macrophages and the activity scores, degree of proteinuria, or lupus disease activity. Urinary CD11c⁺ macrophages were more abundant in patients who did not achieve renal response to induction treatment with immunosuppressants than in those who achieved complete or partial response. The receiver operating characteristic (ROC) curve analysis showed that the number of urinary CD11c⁺ macrophages was the most powerful predictor of renal response at 6 months (ROC-AUC = 1.00, p = 0.0004).

Conclusion

The urinary levels of CD11c⁺ macrophages were closely associated with the chronic pathologic changes of LN and renal response and may thus be used as a novel biomarker in LN.

SUBMITTER: Kim J

PROVIDER: S-EPMC7368794 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Publications

Clinical and histological significance of urinary CD11c<sup>+</sup> macrophages in lupus nephritis.

Kim Jihye J Lee Jung Sun JS Go Heounjeong H Lim Joon Seo JS Oh Ji Seon JS Kim Yong-Gil YG Lee Chang-Keun CK Yoo Bin B Hong Seokchan S

Arthritis research & therapy 20200717 1

<h4>Background</h4>Infiltration of immune cells into the kidney is one of the key features of lupus nephritis (LN). The presence of immune cells in the urine may be used as a non-invasive biomarker of LN. Here, we aimed to analyze the clinicopathologic significance of urinary CD11c<sup>+</sup> macrophages in patients with LN.<h4>Methods</h4>The numbers and proportions of CD11c<sup>+</sup> macrophages in the urine samples of patients with LN at the time of kidney biopsy were examined using flow c ...[more]

PMID: 32680578

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Project description:IntroductionSystemic lupus erythematosus (SLE or lupus) is a chronic autoimmune disease, and kidney involvement with SLE, a.k.a. lupus nephritis (LN), is a frequent and severe complication of SLE that increases patient morbidity and mortality. About 50% of patients with SLE encounter renal abnormalities which, if left untreated, can lead to end-stage renal disease. Kidney biopsy is considered the criterion standard for diagnosis and staging of LN using the International Society of Nephrology/Renal Pathology Society (ISN/RPS) classification, which was developed to help predict renal outcomes and assist with medical decision-making. However, kidney biopsy-based classification of LN is highly invasive and impractical for real-time monitoring of LN status. Here, nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling was used to identify urinary metabolites that discriminated between proliferative and pure membranous LN as defined by the ISN/RPS classification, and between LN and primary focal segmental glomerulosclerosis (FSGS).MethodsMetabolic profiling was conducted using urine samples of patients with proliferative LN without membranous features (Class III/IV; n = 7) or pure membranous LN (Class V; n = 7). Patients with primary FSGS and proteinuria (n = 10) served as disease controls. For each patient, demographic information and clinical data was obtained and a random urine sample collected to measure NMR spectra. Data and sample collection for patients with LN occurred around the time of kidney biopsy. Metabolic profiling analysis was done by visual inspection and principal component analysis.ResultsUrinary citrate levels were 8-fold lower in Class V LN compared to Class III/IV patients, who had normal levels of urinary citrate (P < 0.05). Class III/IV LN patients had > 10-fold lower levels of urinary taurine compared to Class V patients, who had mostly normal levels (P < 0.01). Class V LN patients had normal urinary hippurate levels compared to FSGS patients, who completely lacked urinary hippurate (P < 0.001).ConclusionsThis pilot study indicated differences in urinary metabolites between proliferative LN and pure membranous LN patients, and between LN and FSGS patients. If confirmed in larger studies, these urine metabolites may serve as biomarkers to help discriminate between different classes of LN, and between LN and FSGS.

Dataset Information

Clinical and histological significance of urinary CD11c⁺ macrophages in lupus nephritis.

Background

Methods

Results

Conclusion

Publications

Clinical and histological significance of urinary CD11c<sup>+</sup> macrophages in lupus nephritis.

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Dataset Information

Clinical and histological significance of urinary CD11c+ macrophages in lupus nephritis.

Background

Methods

Results

Conclusion

Publications

Clinical and histological significance of urinary CD11c<sup>+</sup> macrophages in lupus nephritis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Clinical and histological significance of urinary CD11c⁺ macrophages in lupus nephritis.