Dataset Information

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics.

ABSTRACT: Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue light quantity (fluence rate) is important for plant life, few studies have focused on the effects of different blue light intensity on plant secondary metabolism regulation, including tea plants. Here, we performed transcriptomic and metabolomic analyses of young tea shoots (one bud and two leaves) under three levels of supplemental blue light, including low-intensity blue light (LBL, 50 ?mol m-2 s-1), medium-intensity blue light (MBL, 100 ?mol m-2 s-1), and high-intensity blue light (HBL, 200 ?mol m-2 s-1). The total number of differentially expressed genes (DEGs) in LBL, MBL and HBL was 1, 7 and 1097, respectively, indicating that high-intensity blue light comprehensively affects the transcription of tea plants. These DEGs were primarily annotated to the pathways of photosynthesis, lipid metabolism and flavonoid synthesis. In addition, the most abundant transcription factor (TF) families in DEGs were bHLH and MYB, which have been shown to be widely involved in the regulation of plant flavonoids. The significantly changed metabolites that we detected contained 15 lipids and 6 flavonoid components. Further weighted gene co-expression network analysis (WGCNA) indicated that CsMYB (TEA001045) may be a hub gene for the regulation of lipid and flavonoid metabolism by blue light. Our results may help to establish a foundation for future research investigating the regulation of woody plants by blue light.

SUBMITTER: Wang P

PROVIDER: S-EPMC7369854 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics.

Wang Pengjie P Chen Sirong S Gu Mengya M Chen Xiaomin X Chen Xuejin X Yang Jiangfan J Zhao Feng F Ye Naixing N

International journal of molecular sciences 20200629 13

Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue light quantity (fluence rate) is important for plant life, few studies have focused on the effects of different blue light intensity on plant secondary metabolism regulation, including tea plants. Here, we performed transcriptomic and metabolomic analyses of young tea shoots (one bud and two leaves) under three levels of supplemental blue light, including low-intensity blue light ( ...[more]

PMID: 32610479

Similar Datasets

Project description:Functional constituents in the leaves of Passiflora plants contain antidepressant and antianxiety effects which are beneficial to human health and fitness. The objective of this study was to investigate leaf growth, physiological parameters, and secondary metabolite contents of Tainung No. 1 variety (P. edulis × P. edulis f. flavicarpa.) and P. suberosa in response to three light intensity conditions, including 100% light intensity (LI-100), 50% light intensity (LI-50), and 15% light intensity (LI-15) for 2 months. The leaf number, length, width, area, dry weight (DW), minimal fluorescence (Fo), maximal fluorescence (Fm), maximum photochemical efficiency of photosystem II, and soil-plant analysis development (SPAD) values of all tested plants increased with a decreasing light intensity, except for the leaf number and DW of P. suberosa plants. Low values of the net photosynthetic rate, transpiration rate, and stomatal conductance of Tainung No. 1 leaves in the LI-15 treatment showed the acclimation capacity of these plants. These observations together with high values of leaf growth traits of Fo, Fm, SPAD, and the intercellular-to-atmospheric CO2 concentration ratio indicate their physiological plasticity, which is of fundamental importance when cultivating plants in environments with different light availabilities. Wide variations occurred in total phenol (TP), total flavonoid (TF), orientin (OR), and isovitexin (IV) contents of the two Passiflora varieties, and P. suberosa contained higher TP and TF contents than did Tainung No. 1 in each light treatment but IV content of P. suberosa was lower than that of Tainung No. 1 in the LI-15 treatment. Moreover, increases in TF, OR, and IV contents of Tainung No. 1 and P. suberosa were clear in the LI-50 and LI-100 treatments, respectively, compared to LI-15 treatment. Leaf growth, physiological parameters, and secondary metabolite accumulations in Passiflora species can be optimized for commercial production via lighting control technologies, and this approach may also be applicable to leafy vegetables to produce a stable industrial supply of high leaf yields and metabolite contents.

Project description:BackgroundSafflower (Carthamus tinctorius L.) is a domesticated species with a long history of cultivation and widespread distribution across the globe, and light plays an important role in controlling its distribution boundary. Flowers from safflower have been widely used in traditional Chinese medicine because of their ability to improve cerebral blood flow. Flavonoids are the main active compounds in safflower and have many pharmacological effects. In this study, we aimed to explore the relationship between different light intensities and flavonoid biosynthesis in safflower flowers cultivated in greenhouse.MethodsThe transcriptome of safflower flowers grown under different light intensities were sequenced through BGISEQ-500 platform. After assembled and filtered, Unigenes were annotated by aligning with seven functional databases. Differential expression analysis of two samples was performed with the DEseq2 package. Differentially expressed genes (DEGs) related with flavonoids biosynthesis were analyzed by Real-time PCR (RT-PCR). Flavonoids accumulation in flowers were determined by high performance liquid chromatography and spectrophotometer.ResultsTranscriptome analysis of safflower flowers cultivated under different light intensities was performed. A total of 99.16 Gb data were obtained, and 78,179 Unigenes were annotated. Among the DEGs, 13 genes were related to flavonoid biosynthesis. The differential expressions of seven key genes were confirmed by RT-PCR. In addition, the levels of some flavonoids were measured in safflower flowers grown under different light intensities. CtHCT3 gene expression showed a significantly negative correlation with kaempferol content in safflower grown under different light intensities.ConclusionOur results strongly suggested that the reduction in light intensity in a suitable range promoted flavonoid biosynthesis in safflower flowers. We suggest that the expressions of HCT genes played an important role in flavonoid accumulation in safflower flowers. Our study lays a foundation for further research on the effects of light on flavonoid biosynthesis in safflower.

Project description:LED illumination can have a narrow spectral band; its intensity and time regime are regulated within a wide range. These characteristics are the potential basis for the use of a combination of LEDs for plant cultivation because light is the energy source that is used by plants as well as the regulator of photosynthesis, and the regulator of other physiological processes (e.g., plant development), and can cause plant damage under certain stress conditions. As a result, analyzing the influence of light spectra on physiological and growth characteristics during cultivation of different plant species is an important problem. In the present work, we investigated the influence of two variants of LED illumination (red light at an increased intensity, the "red" variant, and blue light at an increased intensity, the "blue" variant) on the parameters of photosynthetic dark and light reactions, respiration rate, leaf reflectance indices, and biomass, among other factors in lettuce (Lactuca sativa L.). The same light intensity (about 180 µmol m-2s-1) was used in both variants. It was shown that the blue illumination variant increased the dark respiration rate (35-130%) and cyclic electron flow around photosystem I (18-26% at the maximal intensity of the actinic light) in comparison to the red variant; the effects were dependent on the duration of cultivation. In contrast, the blue variant decreased the rate of the photosynthetic linear electron flow (13-26%) and various plant growth parameters, such as final biomass (about 40%). Some reflectance indices (e.g., the Zarco-Tejada and Miller Index, an index that is related to the core sizes and light-harvesting complex of photosystem I), were also strongly dependent on the illumination variant. Thus, our results show that the red illumination variant contributes a great deal to lettuce growth; in contrast, the blue variant contributes to stress changes, including the activation of cyclic electron flow around photosystem I.

Dataset Information

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics.

Publications

Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets