A Co-registration Pipeline for Multimodal MALDI and Confocal Imaging Analysis of Stem Cell Colonies.
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ABSTRACT:
SUBMITTER: Nikitina A
PROVIDER: S-EPMC7370321 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Publications
A Co-registration Pipeline for Multimodal MALDI and Confocal Imaging Analysis of Stem Cell Colonies.
Nikitina Arina A Huang Danning D Li Li L Peterman Nicholas N Cleavenger Sarah E SE Fernández Facundo M FM Kemp Melissa L ML
Journal of the American Society for Mass Spectrometry 20200319 4
Multimodal mass spectrometry imaging (MSI) data presents unique big data challenges in handling and analysis. Here, we present a pipeline for co-registering matrix-assisted laser desorption/ionization MSI and confocal immunofluorescence imaging data for extracting single-cell metabolite signatures. We further describe methods and introduce software for the simultaneous analysis of these concatenated data sets, which are designed to establish a connection between cell traits of interest (shape me ...[more]
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