Analysis of long non-coding RNA expression profiles in high-glucose treated vascular endothelial cells.

ABSTRACT: BACKGROUND:Diabetes mellitus is often associated with microvascular and macrovascular lesions, and hyperglycemia-induced vascular endothelial cell damage is a key factor. METHODS:We investigated long non-coding RNAs (lncRNAs) and mRNAs that are affected by hyperglycemia-induced damage using human umbilical vein endothelial cells (HUVECs) as a model. HUVECs were cultured under high (25?mmol/L) or normal (5?mmol/L) glucose conditions for 6 d, and then lncRNAs and protein-coding transcripts were profiled by RNA-seq. RESULT:Among 40,379 lncRNAs screened, 214 were upregulated (log2 [fold-change]?>?1, FDR?

SUBMITTER: Xu E 

PROVIDER: S-EPMC7372841 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature