Unknown

Dataset Information

0

A Novel Multiplex-PCR Assay to Detect Three Non-Halal Meats Contained in Meatball using Mitochondrial 12S rRNA Gene.

ABSTRACT: The objective of this study was to detect three non-halal meat products consisted of dog, pork, and rat species in meatball using novel multiplex-PCR with 12S rRNA gene as target sites. A total of 33 self-made meatballs were used, and they were grouped into eleven types of meatball based on meat species origin contained in the meatballs. Each type consisted of three meatballs. Extraction of genomic DNA from the meatballs was used as a DNA template for simplex-, duplex-, and multiplex-PCR processes. The result of simplex-PCR, duplex-PCR, and multiplex-PCR showed that the 12S rRNA primer gene successfully amplified DNA for each species bovine, dog, pig, and rat, which are respectively indicated by 155, 244, 357, and 491 bp of DNA bands. In addition, multiplex-PCR with 12S rRNA gene primers can be uniquely and accurately used for detection bovine, dog, pig, and rat species on beef meatball in one reaction.

SUBMITTER: Cahyadi M 

PROVIDER: S-EPMC7372981 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A Novel Multiplex-PCR Assay to Detect Three Non-Halal Meats Contained in Meatball using Mitochondrial 12S rRNA Gene.

Cahyadi Muhammad M   Wibowo Tommy T   Pramono Ahmad A   Abdurrahman Zakaria Husein ZH  

Food science of animal resources 20200701 4

The objective of this study was to detect three non-halal meat products consisted of dog, pork, and rat species in meatball using novel multiplex-PCR with 12S rRNA gene as target sites. A total of 33 self-made meatballs were used, and they were grouped into eleven types of meatball based on meat species origin contained in the meatballs. Each type consisted of three meatballs. Extraction of genomic DNA from the meatballs was used as a DNA template for simplex-, duplex-, and multiplex-PCR process  ...[more]

Similar Datasets

Unknown Screening for deafness-associated mitochondrial 12S rRNA mutations by using a multiplex allele-specific PCR method.
| S-EPMC7263198 | biostudies-literature
Unknown Development of a novel multiplex PCR assay to detect functional subtypes of KIR3DL1 alleles.
| S-EPMC4053526 | biostudies-literature
Unknown Multiplex real-time PCR assay to detect illegal trade of CITES-listed shark species.
| S-EPMC6218538 | biostudies-literature
Unknown Multiplex PCR assay to detect high risk lineages of Salmonella Typhi and Paratyphi A.
| S-EPMC9307194 | biostudies-literature
Unknown A Novel Multiplex qRT-PCR Assay to Detect SARS-CoV-2 Infection: High Sensitivity and Increased Testing Capacity.
| S-EPMC7409169 | biostudies-literature
Unknown A Multiplex RT-PCR Assay to Detect and Discriminate Porcine Reproductive and Respiratory Syndrome Viruses in Clinical Specimens.
| S-EPMC5580462 | biostudies-literature
Unknown A rapid, multiplex digital PCR assay to detect gene variants and fusions in non-small cell lung cancer.
| S-EPMC10620117 | biostudies-literature
Unknown Associations between GJB2, mitochondrial 12S rRNA, SLC26A4 mutations, and hearing loss among three ethnicities.
| S-EPMC3996913 | biostudies-literature
Unknown A Novel Multiplex HRM Assay to Detect Clopidogrel Resistance.
| S-EPMC5700108 | biostudies-literature
Unknown Validation of multiplex PCR sequencing assay of SIV.
| S-EPMC7810186 | biostudies-literature