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GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD.

ABSTRACT: Cystogenesis is a morphological consequence of numerous genetic diseases of the epithelium. In the kidney, the pathogenic mechanisms underlying the program of altered cell and tubule morphology are obscured by secondary effects of cyst expansion. Here, we developed a new 3D tubuloid system to isolate the rapid changes in protein localization and gene expression that correlate with altered cell and tubule morphology during cyst initiation. Mouse renal tubule fragments were pulsed with a cell differentiation cocktail including glial-derived neurotrophic factor (GDNF) to yield collecting duct-like tubuloid structures with appropriate polarity, primary cilia, and gene expression. Using the 3D tubuloid model with an inducible Pkd2 knockout system allowed the tracking of morphological, protein, and genetic changes during cyst formation. Within hours of inactivation of Pkd2 and loss of polycystin-2, we observed significant progression in tubuloid to cyst morphology that correlated with 35 differentially expressed genes, many related to cell junctions, matrix interactions, and cell morphology previously implicated in cystogenesis.This article has an associated First Person interview with the first author of the paper.

SUBMITTER: Dixon EE

PROVIDER: S-EPMC7375472 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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GDNF drives rapid tubule morphogenesis in a novel 3D <i>in vitro</i> model for ADPKD.

Dixon Eryn E EE Maxim Demetrios S DS Halperin Kuhns Victoria L VL Lane-Harris Allison C AC Outeda Patricia P Ewald Andrew J AJ Watnick Terry J TJ Welling Paul A PA Woodward Owen M OM

Journal of cell science 20200716 14

Cystogenesis is a morphological consequence of numerous genetic diseases of the epithelium. In the kidney, the pathogenic mechanisms underlying the program of altered cell and tubule morphology are obscured by secondary effects of cyst expansion. Here, we developed a new 3D tubuloid system to isolate the rapid changes in protein localization and gene expression that correlate with altered cell and tubule morphology during cyst initiation. Mouse renal tubule fragments were pulsed with a cell diff ...[more]

PMID: 32513820

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GDNF drives rapid tubule morphogenesis in a novel 3D in vitro model for ADPKD.

Publications

GDNF drives rapid tubule morphogenesis in a novel 3D <i>in vitro</i> model for ADPKD.

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