Molecular detection and genetic characteristics of Babesia gibsoni in dogs in Shaanxi Province, China.

ABSTRACT:

Background

Several members of genus Babesia are important pathogens causing babesiosis in dogs. In China, at least five Babesia species have been described in dogs or ticks. This study sought to determine the prevalence and molecular characteristics of various Babesia spp. in dogs in cities in Shaanxi Province in China, including Xi'an and Hanzhong.

Methods

A total of 371 blood samples were collected from pet dogs presenting to veterinary clinics in the cities of Xi'an and Hanzhong in Shaanxi, China. Babesia spp. DNA was detected via amplification of partial 18S rRNA genes by semi-nested PCR. Almost full-length 18S rRNA, ITS, partial TRAP and complete cytb genes were recovered for analysis of the genetic characteristics and relationships with known isolates.

Results

A

SUBMITTER: Guo WP 

PROVIDER: S-EPMC7376908 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature